A. Derakhshandeh
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
[ 1 ] - Characterization of Salmonellae isolated from different animal and human sources by PCR and resistance trends
Salmonella is one of the most important zoonotic pathogens with many virulence factors playing a major role in its pathogenesis. The aims of this study were to detect spvA, int2 and invC virulence genes of different Salmonella serotypes isolated from various clinical animal and human sources, and to investigate antibiotic resistance patterns among these serotypes. Using a PCR assay, a total of ...
[ 2 ] - Phylogenetic group determination of faecal Escherichia coli and comparative analysis among different hosts
Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2 and D). Characterization of phylogenetic groups is of clinical interest, as group A and B1 generally associated with commensals, whereas most enteropathogenic isolates are assigned to group D, and group B2 is associated with extra-intestinal pathotype. One hundred E. coli strains recove...
[ 3 ] - In silico design and expression of a novel fusion protein of HBHA and high antigenic region of FAP-P of Mycobacterium avium subsp. paratuberculosis in Pichia pastoris
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne's disease in ruminants and there has been a shift in the public health approach to MAP and human diseases like Crohn's disease. The prevention of infection by MAP in ruminants is thought to deter the high impact of economic losses in the level of dairy industry and possible spreading of this pathogen in dairy prod...
[ 4 ] - Phylogenetic analysis of Escherichia coli strains isolated from human samples
Escherichia coli (E. coli) is a normal inhabitant of the gastrointestinal tract of vertebrates, including humans. Phylogenetic analysis has shown that E. coli is composed of four main phylogenetic groups (A, B1, B2 and D). Group A and B1 are generally associated with commensals, whereas group B2 is associated with extra-intestinal pathotypes. Most enteropathogenic isolates, however, are assigne...
[ 5 ] - Detection of Mycobacterium avium subspecies paratuberculosis infection in two different camel species by conventional and molecular techniques
Paratuberculosis (John’s disease) is infectious and chronically progressive granulomatous disease which affects domestic and wild ruminants. The causative agent is Mycobacterium avium paratuberculosis (MAP), a slow growing mycobactin dependent acid-fast bacillus. We investigated the detection and frequency of MAP in apparently healthy dromedary and Bactrian camels by insertion...
[ 6 ] - همراهی چندریختی در ژنهای DGAT1 و IGF-I با عملکرد تولیدی و تولیدمثلی در گاوهای هلشتاین
زمینه مطالعاتی: هدف پژوهشهای ژنومیک در گاوهای شیری، شناسایی ژنهای کنترل کنندهی صفات اقتصادی است که در برنامههای اصلاحنژادی کاربرد دارند. هدف: هدف این پژوهش بررسی همراهی چندریختی در اگزون 8 ژن DGAT1 و اگزون 4 ژن IGF-I با عملکرد تولیدی و تولیدمثلی در یک نمونه از گاوهای هلشتاین بود. روش کار: در این پژوهش از 133 گاو خونگیری شد و پس از استخراج DNA ژنومیک آنها، ژنهای مورد مطالعه با استفاده ا...
[ 7 ] - Goat paratuberculosis in Shiraz: Histopathological and molecular approaches
In the present study, Mycobacterium avium subsp. paratuberculosis (MAP) was investigated in goats slaughtered in Shiraz abattoir using histopathological examinations and polymerase chain reaction (PCR). Ilium and mesenteric lymph node samples from 66 suspected goat carcasses to Johne’s disease were collected. Among 66 examined slaughtered goats, nine (13.63%) goats were positi...
[ 8 ] - مقایسه مقاومت آنتی‌بیوتیکی و فراوانی ژنهای حدت در اشریشیا کلی‌های ادراری و مدفوعی افراد مبتلا به عفونت دستگاه ادراری
زمینه و هدف: عفونت‌های دستگاه ادراری یکی از مهم‌ترین عفونت‌های باکتریایی هستند و شایع‌ترین عامل این عفونت‌ها باکتری اشریشیا کلی می‌باشد. با توجه به اهمیت اشریشیا کلی‌های فلور طبیعی دستگاه گوارش در ایجاد عفونت ادراری، مطالعه حاضر به منظور مقایسه اشریشیا کلی‌های عامل عفونت دستگاه ادرای با اشریشیا کلی‌های غالب فلور طبیعی دستگاه گوارش براساس مقاومت آنتی‌بیوتیکی و برخی ژنهای حدت انجام گرفت. روش...
[ 9 ] - Cloning and characterization of MAP2191 gene, a mammalian cell entry antigen of Mycobacterium avium subspecies paratuberculosis
The aim of this study is to identify, clone and express a Mycobacterium avium subsp. paratuberculosis specific immunogenic antigen candidate, in order to develop better reagents for diagnosis and vaccines for the protection of the host. Therefore, MAP2191 gene (a member of MAPmce5 operon) from MAP, was isolated and characterized by Bioinformatics tools and <e...
[ 10 ] - A novel chimeric recombinant protein PDHB-P80 of Mycoplasma agalactiae as a potential diagnostic tool
The aim of this study was to construct, expression of a novel recombinant chimeric protein consisting of Pyruvate dehydrogenase beta subunit (PDHB) and high antigenic region of integral membrane lipoprotein P80 of Mycoplasma agalactiae as a potential diagnostic tool. The full-length sequence of pdhb and a portion of antigenic regions of P80 were selected and analyzed by CLC ma...
[ 11 ] - A comparative study of the prevalence of extended-spectrum β-lactamaseproducing Escherichia coli among urinary and fecal Escherichia coli isolates of women with urinary tract infection in Kerman city
Background & Aim: Important challenge for treatment of urinary tract infections is the spread of extended-spectrum beta-lactamases (ESBLs) producing Escherichia coli. Therefore, the frequencies of main beta-lactamase genes, resistance to beta-lactams and the ability of ESBLs production in urinary and fecal Escherichia coli of women with urinary tract infections were compared. Methods: In 60 Es...
نویسندگان همکار
S, Zare 1