عطایی, رمضانعلی

گروه میکروب‌شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی بقیه‌ا...(عج)، تهران، ایران

[ 1 ] - معرفی روش کاربردی در تدریس واحد سمینار دوره کارشناسی‌ارشد

اهداف: واحد سمینار کارشناسی‌ارشد در بسیاری از رشته‌های علوم پایه پزشکی به‌صورت سلیقه‌ای برگزار می‌شود و به روش‌های معتبر بهره‌گیری از منابع و توانمندسازی دانشجو توجه نمی‌گردد. هدف این پژوهش ارایه روشی کاربردی برای برگزاری درس سمینار دانشجویان کارشناسی‌ارشد بود. روش‌ها: در یک مطالعه مقطعی توصیفی طی سالهای 1387 الی 1390، تمامی دانشجویان در دسترس رشته توکسین‌های میکروبی و میکروب‌شناسی وارد پژوهش ش...

[ 2 ] - تشخیص استرپتوکوکوس پنومونیه بر اساس ژن‌های cpsB ، cpsD و psaA

Background and purpose: Fast diagnosis of pneumococcal infections is of great importance in achieving successful outcomes. The aim of this study was to use PCR process based on capsular encoding genes of Streptococcus pneumonia. Materials and methods: In this experimental study, specific primers were designed based on the reference sequences of the Streptococcus pneumoniae cpsB, cpsD, aroE, an...

[ 3 ] - Molecular Detection of Bacterial Etiology of Rheumatoid Arthritis

Background and purpose: Etiology of rheumatoid arthritis is not fully recognized. The purpose of this study was to use universal and specific primers to trace bacteria in the blood and synovial fluid in patients with rheumatoid arthritis. Materials and methods: In this experimental study, a PCR method was developed to identify a wide range of bacteria in general and Staphylococcus aureus in sp...

[ 4 ] - Real-time RT PCR Evaluation of the Xylitol Effect on the Expression of Streptococcus pneumoniae cpsB,cpsD and psaA Genes

Background and Objective: The major pneumococcal pathogenesis factor is the capsular polysaccharide. the production of polysaccharide is regulated by cpsB and cpsD genes. Thus, every agent that induce or inhibition of expression of these genes probably increased pathogenesis of bacteria, intracellular survival and vis versa. The aim of this study was to assay the effect of Xylitol on Streptococ...

[ 5 ] - Specific Markers of Staphylococcus aureus and Escherichia coli VBNC in Patients with Rheumatoid Arthritis

Background and purpose: Recent studies suggest that the viable but nonculturable (VBNC) bacteria could cause inflammatory diseases. The aim of this study was to detect and investigate the expression of nuc and rfbE genes in blood samples of patients with rheumatoid arthritis (RA). Materials and methods: In this experimental study, 102 blood samples were collected from patients with RA and univ...

[ 6 ] - Real- Time - RT PCR Study of Aqueous Extract of Ammi visnaga on Pseudomonas aeruginosa Exo A and Exo S Genes Expression

Background and Aim: P. aeruginosa exotoxins are responsible for pathogenicity and antibiotic resistant. The aim of this study was to investigate the effects of the Ammi. visnaga (A. visnaga) extract on the P. aeruginosa exotoxin A and S genes expression. Materials and Methods: Antibacterial effects of the A. visnaga ethanolic extract were performed using the micro dilution technique in sequent...