In order to provide useful genomic information for agronomical plants, we have established a database, the Kazusa Marker DataBase (http://marker.kazusa.or.jp). This database includes information on DNA markers, e.g., SSR and SNP markers, genetic linkage maps, and physical maps, that were developed at the Kazusa DNA Research Institute. Keyword searches for the markers, sequence data used for mar...

The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different concentrations of BAP and NAA as growth regulators. The best shoot formation ha...

The essential oils of three different growth stages of Trifolium pratense L. (TP1, TP2 and TP3) were investigated by gas chromatography-mass spectrometry and tested for their antioxidant and antimicrobial activities. The highest content of volatile compounds was found in the essential oil sample TP1, where terpenes such as β-myrcene (4.55%), p-cymene (3.59%), limonene (0.86%), tetrahydroionone ...

Organochlorine pesticides were tested in order to establish optimal conditions for rapid detection in dried leafy matrices, using DRS software. In tests we have used dried Trifolium pratense herba fortified with OC pesticide mix, processed with modified QuEChERS method. Instrumental analysis was carried out complying Agilent "key" condition, column of 15 m, in which case the retention time for ...