Efflux pumps are membrane transporters that actively extrude various substrates, leading to multidrug resistance (MDR). In this study, we have designed a new test that allows investigating the assembly of the MexA-MexB-OprM efflux pump from the Gram negative bacteria Pseudomonas aeruginosa. The method relies on the streptavidin-mediated pull-down of OprM proteoliposomes upon interaction with Me...

Recently, multidrug-resistant pathogens have disseminated widely owing essentially to their increased multidrug efflux pump activity. Presently, there is a scarcity of new antibacterial agents, and hence, inhibitors of multidrug efflux pumps belonging to the resistance-nodulation-cell division (RND) family appear useful in the treatment of infections by multidrug-resistant pathogens. Moreover, ...

Pseudomonas aeruginosa OprM is a protein involved in multiple-antibiotic resistance as the outer membrane component for the MexA-MexB-OprM efflux system. Planar lipid bilayer experiments showed that OprM had channel-forming activity with an average single-channel conductance of only about 80 pS in 1 M KCl. The gene encoding OprM was subjected to insertion mutagenesis by cloning of a foreign epi...

For any non-negative integers a and b, we consider the following game WY T (a, b). Given two piles that consist of x and y matches, two players alternate turns; a single move consists of a player choosing x′ matches from one pile and y′ from the other, such that 0 ≤ x′ ≤ x, 0 ≤ y′ ≤ y, 0 < x′ + y′, and [min(x′, y′) < b or |x′ − y′| < a]. The player who takes the last match is the winner in the ...

Pseudomonas aeruginosa infections are becoming increasingly difficult to treat due to intrinsic antibiotic resistance and the propensity of this pathogen to accumulate diverse resistance mechanisms. Hyperexpression of efflux pumps of the Resistance-Nodulation-Cell Division (RND)-type multidrug efflux pumps (e.g., MexAB-OprM), chromosomally encoded by mexAB-oprM, mexCD-oprJ, mexEF-oprN, and mexXY (-...

Of the nine efflux systems of the RND (resistance nodulation cell division) family characterized so far in Pseudomonas aeruginosa, four confer significant resistance to antibiotics when overproduced. These systems, namely, MexAB-OprM (ABM), MexCD-OprJ (CDJ), MexEF-OprN (EFN), and MexXY (XY), are individually able to pump out multiple antipseudomonal compounds, including -lactams (ABM, CDJ, XY),...