Lactic acid concentrations increased in refrigerated and freeze-thawed anaerobically stored ground beef. Bacterial counts were higher in refrigerated samples, but the ratios of gram-positive bacteria in refrigerated and freeze-thawed samples were the same. No differences in appearance or odor between refrigerated and freeze-thawed samples were noted after 2 days of aerobic storage. Initial lact...

From Jan 12th to Feb 12th 2008, the most severity cold chilling and freeze injury weather took place during the last 50 years in the southern of China. Sugar-cane was suffered injury severity. However, the losing of sugar-cane which it was aroused by this weather disaster had not been exactitude evaluated till on Apr 1st, 2008. It was not only affected the sugar-cane ordinary harvesting and cru...

The effects of storage temperatures, repeated freeze-thaw cycles, or delays in separating plasma or serum from blood samples are largely unknown for heat shock protein 27 (HSP27). We evaluated (1) the imprecision of the HSP27 assay used in this study; (2) the in vitro stability of HSP27 in blood samples stored at 4°C for up to 6 hr with immediate and delayed serum/plasma separation from cells; ...

The timing and location of microcracking events, their propagation coalescence to form macrocracks, development by tension, shearing or mixed modes are little known but essential understanding the fracture intact rock freezing thawing. aims present study investigate mechanisms transition macrocracking during repeated freeze-thaw, develop a statistical model crack that assesses distance angular ...

Freeze-dried mouse spermatozoa can be used for normal embryonic development after injection into oocytes, thus indicating that freeze-drying is a useful method for the storage and transportation of genetic materials from animals. We recently reported that storage of freeze-dried mouse spermatozoa requires maintenance at temperatures lower than -80 C for long-term preservation and a pressure of ...

Freeze-drying (lyophilization) has been proposed as an alternative method for sperm preservation to overcome the disadvantages of the current cryopreservation method such as the high maintenance cost of frozen stocks, the problems associated with transportation of frozen materials and the potential risk of total loss of the frozen stock. Since freeze-dried spermatozoa after rehydration lose the...

objective(s):one of the major challenges in the field of vaccine design is choosing immunogenic antigens which can induce a proper immune response against complex targets like malignant cells or recondite diseases caused by protozoan parasites such as leishmaniasis. the aim of this study was to find a way to construct artificial liposome-based cells containing fragments of target’s cell membran...

Sperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new preservation method has been developed that enables sperm to be stored for a long time in a refrigerator at 4 °C. Sperm are freeze-dried in a solution containing 10 mM Tris and 1 mM EDTA. Using this method, liquid nitrogen is not required for the ...

SYNOPSIS The effects of nursery practices on freeze tolerance of P taeda and P elliottii seedlings were examined by placing the seedlings in a freezing chamber and lowering the temperature to -10C. Injury was evaluated 10 days after the freeze treatment by examining cambial tissue (browning test). Top-pruning P taeda seedlings (three times) increased freeze tolerance while rootwrenching (six ti...