ment [4–6]. This prevalence is high if we consider that only 5–10% of WG patients were shown to have CNS disease in large cohorts [6]. Therefore, ANCA-negative WG may hide remarkable CNS involvement [5]. In our patient, WG initially presented as a systemic disease and C-ANCA (anti-PR3) tested positive using routine IIF and direct-ELISA. At the time WG relapsed with upper airway tract and mening...

Arsenic-induced carcinogenesis can result in cancers of the liver exposed organisms. This study evaluated anticancer potentials MLF1 and AMF1 extracted from Morinda lucida Annona muicata leaves respectively Sodium arsenite (SA)-induced toxicity rats. Sixty adult female rats were randomly divided into 12 groups (n = 5). Group 1 was control. 2 received 5-weeks administrations 10 mg/kg bodyweight ...

OBJECTIVES To investigate and characterize Mycobacterium avium subspecies paratuberculosis (MAP) in patients with Crohn's disease, attendants of animals with suspected infection, and healthy humans, using multiple diagnostic tests. METHODS A total of 119 samples (35 stool, 76 serum, three blood clots, and five biopsies) were collected from five patients with Crohn's disease, eight attendants ...

ECR Spotlight is a series of interviews with early-career authors from selection papers published in Journal Experimental Biology and aims to promote not only the diversity researchers (ECRs) working experimental biology during our centenary year but also huge variety animals physiological systems that are essential for ‘comparative’ approach. Elisa Thoral an author on ‘ Temperature-dependent m...

Aim: To study the seroprevalence of caprine bruellosis in Karnataka and compare the relative sensitivity and specificity among the different serological tests used. Materials and Methods: A total of 252 serum samples were collected from the goats of Karnataka and subjected to 5 different serological tests, i.e., Rose Bengal Plate Test (RBPT), Standard Tube Agglutination Test (STAT), 2-mercaptoe...

We investigated the sero-prevalence of toxocariasis among healthy Korean adults in 1999. A total of 314 sera from normal inhabitants in Whachon-gun, Gangwondo, Korea was examined for specific antibody levels against excretory-secretory products of second stage larvae of Toxocara (TES). The presence of cross-reactions with other helminthiases such as cysticercosis, paragonimiasis, sparganosis or...

Enzyme-linked immunosorbent assays (ELISA) for the detection of specific immunoglobulin G (IgG) and IgM antibodies were developed by using purified Coxiella burnetii cells. Variables, including type of microtiter plate, blocking agent, incubation conditions, antigen stability, and substrate type, were examined to achieve optimal ELISA performance. The reliabilities of the assay systems were com...

The hepatitis C antibody reactivity rate in 91,748 blood donors tested using the ORTHO HCV C-100 ELISA system was 0.51%. Specificity of ELISA positive reactions was measured using a recombinant immunoblot assay (RIBA). The aim of this study was to identify markers in ELISA positive donors which were predictive of a RIBA positive result. Samples from 430 ELISA positive donors were tested by the ...

A double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) is described for detection of porcine circovirus 2 (PCV2) antibodies using the well-characterized recombinant PCV2 capsid protein. In a comparative test of 394 pig sera against an indirect immunofluorescence (IIF) test and a commercial ELISA kit (also based on the recombinant PCV2 capsid protein), the results showed that the di...