Fourteen primiparous Holstein cows (180.0 ± 4.3 days in milk) with a mean (±SD) daily milk yield of 35.0 ± 1.3 kg and a body condition score of 3.2 ± 0.2 were allotted into two groups (n = 7), and were fed a ration consisting of (dry matter basis) 35% corn silage, 25% alfalfa hay and 40% concentrates. Salt was provided free choice. The experiment was performed during the period of heat stress, ...

To investigate uterine fluid and serum calcium (Ca) and Magnesium (Mg) variations during the estrus cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by examining ovarian structures. 18 animals were pro-estrous, 15 estrous, 16 met-estrous and 22 diestrous. The uterine fluid was collected by gent...

Milk and blood serum from clinically mastitis infected, subclinically mastitis infected and healthy Friesian cows (15 samples from each of 3 groups) were evaluated for macrominerals (sodium, potassium, calcium, magnesium and phosphorus). The milk from cows infected with subclinical mastitis revealed a significant decrease in potassium (P < 0.001) and a significant increase in sodium and phospho...

Cytokines, eicosanoids, and lactoferrin are involved in the mammary gland's immune response to invading microorganisms. The goal of this work was to investigate the synthesis of these immunologically important factors in somatic milk cells, blood cells, and mammary tissue of cows with different somatic cell count levels, i.e., different immunological activity. On the level of mRNA expression, t...

This study was conducted to investigate individual metabolic and endocrine adaptation to lactation under conditions of identical housing and feeding conditions in high-yielding dairy cows. Forty-five cows were studied on a research farm under standardized but practical conditions. From wk 2 before calving until wk 14 postpartum, blood samples were collected at weekly intervals and assayed for b...

Dairy farms in some arid areas around the world have to use drinking water that contained elevated total dissolved solids (TDS); however, very limited data is available concerning water TDS effects on health status and blood mineral levels of cattle. The aim of this study was to compare 3 dairy cattle groups in several dairy farms with different drinking water TDS: High (HTDS; >4000 ppm), Mediu...

Most dairy cows suffer uterine microbial contamination postpartum. Persistent endometritis often develops, associated with reduced fertility. We used a model of differential feeding and milking regimes to produce cows in differing negative energy balance status in early lactation (mild or severe, MNEB or SNEB). Blood hematology was assessed preslaughter at 2 wk postpartum. RNA expression in end...

The present study was carried out in a herd with concurrent infections of Mycoplasma wenyonii and 'Candidatus M. haemobos', to investigate if transplacental and/or vector-borne transmission is possible for one or both bovine haemoplasma species. For this purpose blood samples were collected from 38 mother animals and their newborn calves; as well as from 17 uninseminated cows twice three months...

sixty eight holstein cows, 38 primiparous and 30 multiparous, were employed in a randomized complete block design to evaluate the effects of 2 diets of varied rumine fermentable carbohydrate sources (corn grain (cg) and wheat grain (wg)) on blood metabolites and hormones during periparturint period. primiparous and multiparous cows were blocked by parity. the cows were fed diets as total mixed ...