BACKGROUND Proximal cytoplasmic droplets (PCDs), a remnant of germ cell cytoplasm, are common non-specific morphological defects in bovine semen. This study evaluated the effect of higher percentages of PCDs on the quality of frozen-thawed bovine semen, embryo production and early embryo development. METHODS Three ejaculates from each of five (group 1: PCD ≤ 1%, control) and eight adult Bos i...

Acridine orange is a metachromatic intercalator used extensively in histochemistry to differentiate double- from single-stranded (ds, ss) nucleic acid by the emission of green and red fluorescence, respectively, under ultraviolet light. In the present study we standardised a protocol in order to use acridine orange to detect rotavirus ds RNA in polyacrylamide gels and compared it to silver and ...

Abstract Objective Density gradient centrifugation (DGC) is commonly used for sperm preparation before assisted reproductive technology (ART) procedures. This technique separates superior motile spermatozoa with normal morphology from the total population. However, there still controversy as to effects of this separation on cell DNA integrity which a determining element in process fertilization...

BACKGROUND Arsenic as an environmental toxicant is able to exert malformations in male reproductive system by inducing oxidative stress. Vitamin E (Vit.E) is known as antioxidant vitamin. OBJECTIVE The aim of this study was to investigate the harmful effects of sodium arsenite on sperm parameters and the antioxidant effects of Vit.E on sperm anomalies in sodium arsenite treated rats. MATERI...

Introduction: Spermatozoa selection for Intracytoplasmic Sperm Injection (ICSI) is a paramount factor in the outcome of a fertility treatment cycle. Nature has perfected the selection process by using the cumulus matrix to select spermatozoa that are morphologically and genetically normal. Aim: To determine which method of semen preparation delivers the best results in terms of spermatozoa sele...

Background: Oxidative stress occurs when the oxidative homeostasis is damage. Excessive ROS are generated and this reaction has been proposed to produce marked damage to the structure and function of sperm. Materials and Methods: This study was designed to investigate the protective effects of of American Ginseng against cyclophosphamide - induced damage in the rat sperm. For this experiment, t...

Diagnosis of sperm DNA integrity of semen sample is important for consistently high reproductive efficiency. The conventional parameters of semen analysis take into account morphology, motility, and concentration of spermatozoa in the sample, which are insufficient for evaluation of reproductive potential. Current studies have implicated abnormal organization of genomic material in sperms as a ...

sperm chromatin integrity has been being recognized as an important factor in male fertility. during normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. however, using assisted reproductive techniques, particularly icsi, the natural selection is bypassed. therefore sperm with dna breakage have the opport...

background: one of the affecting factors in disturbance process of spermatogenesis is chemotherapeutic-induced oxidative stress resulted from cyclophosphamide (cp) treatment which leads to diminished sperm quality via interference in spermatogenesis process. objective: this study was conducted to investigate the effects of ethyl pyruvate (ep) in reducing the cp-induced side effects on reproduct...

background: the present study aimed to determine the prevalence and associated risk factors of vaginal trichomoniasis in women referred to gynecologic clinic in benha university hospital, egypt. methods: two hundred female patients enrolled in the study. vaginal samples were obtained from them and examined for t. vaginalis by wet mount, giemsa stain, acridine orange (ao) stain and culture on mo...