1. Adipocytes isolated from rats 6-9 days after adrenalectomy had significantly increased sensitivity to insulin action against noradrenaline-stimulated lipolysis. In the presence of adenosine deaminase there was no significant difference in insulin sensitivity between cells from adrenalectomized and sham-operated rats. 2. Adipocytes from adrenalectomized rats had decreased lipolytic responses ...

Insect-derived growth factor (IDGF) was originally isolated from conditioned medium of NIH-Sape-4 cells derived from flesh fly embryos. Here we demonstrated that IDGF has adenosine deaminase activity. The substrate specificity of IDGF was similar to that of the mammalian cytoplasmic adenosine deaminase. The adenosine deaminase activity of IDGF was shown to be indispensable for its growth factor...

We report here the identification and nucleotide sequence of two transcription termination regions associated with the murine adenosine deaminase gene. One region is situated within or very near exon one and in mouse fibroblasts accounts for more than a 50-fold drop in the abundance of nascent transcripts. This termination region is believed to be involved in the regulation of adenosine deamina...

 ABSTRACT Background: The aims of this study were to assay the activity of adenosine deaminase (ADA) in estrogen receptor positive (MCF-7) and negative (MDA-MB468) breast cancer cell lines. Methods: MDA-MB468 and MCF-7 breast cancer cell lines were cultured in complete medium, striped serum with and without 0.0 1~-LM diethylstilbestrol (DES), complete medium in the presence and absence of 111M ...

Traditionally, Mycobacterium tuberculosis or granulomas have to be evidenced in the pleural cavity in order to establish a diagnosis of tuberculous pleuritis. In recent years, adenosine deaminase has aided tremendously in the diagnosis of tuberculous pleural effusion, often obviating the need for microbiologic or histologic verification of the diagnosis. However, the fact that the sensitivity o...

An improved method was devised for the estimation of serum adenosine deaminase activity. The principle of the method consists in measurement of a liberated ammonia by the action of the deaminase with a direct colorimetric determination of ammonia concentration [Okuda et al.]. The technique is relatively easy to estimate the serum enzyme activity and can be applied for routine clinical use. The ...

Erythrocytes from 1 1 patients with presumptive diagnoses of transient erythroblastopenia of childhood were evaluated retrospectively (six) or prospectively (five) for a possible relationship between erythrocyte adenosine 5’monophosphate aminohydrolase. adenylic acid deaminase (AMP deaminase) activity and intracellular concentrations of adenine nucleotides. Older red blood cell (RBC) cohorts in...