Five years after the introduction of denaturing gradient gel electrophoresis(DGGE) and temperature gradient gel electrophoresis (TGGE) in environmental microbiology these techniques are now routinely used in many microbiological laboratories worldwide as molecular tools to compare the diversity of microbial communities and to monitor population dynamics. Recent advances in these techniques have...

Bauhinia racemosa Lamk. (Caesalpiniaceae) is a small tree where bark and leaves are used extensively for the treatment of inflammation, headache, fever, malaria, skin infections, dysentery and diarrhea. The primary objective of this study was to evaluate the antioxidant, DNA cleavage and α-amylase inhibitory effects of the phenolic compounds: racemosol (BR-I), de-O-methyl racemosol (BR-II), 1,7...

Electrophoretograms of atypical creatine kinase (EC 2.7.3.2, CK) isoenzymes may be difficult to interpret. In addition, their presence may cause discrepancies between results by immunological determinations for mass or activity and those by electrophoresis. Here we outline procedures to use in characterizing apparently atypical CK isoenzymes observed after electrophoresis, as illustrated by a C...

image analysis is an image processing technique that aims to extract features or information from images. image analysis in medicine has a special place because is a basis for disease diagnosis for physicians. electrophoresis is a laboratory separating technique. electrophoresis images are created during the electrophoresis process. serum protein and hemoglobin electrophoresis test are the most...

The proteins synthesized in vaccinia-infected HeLa cells have been analyzed at different times after infection by using two-dimensional gel electrophoresis. Vaccinia-infected cells present up to 198 polypeptides (138 acidic, isoelectric focusing; 60 basic, nonequilibrium pH gradient electrophoresis) not detected in control cells. Cells infected in the presence of cycloheximide show 81 additiona...

Abstract Background Breast cancer, one of the most prevalent cancer types among women worldwide as well in Bangladesh, is leading cause death throughout globe. The risk breast development was found to be associated with genetic polymorphism according several studies. As a convenient prognostic marker, biomarker helps identify disease progression, can lead an effective therapeutic strategy, mark...

Response criteria for multiple myeloma are based upon changes in monoclonal protein levels quantified using serum and/or urine protein electrophoresis. The latter lacks sensitivity at low monoclonal protein levels and since 2001, the serum free light chain test has been available and its clinical utility proven, yet guidelines have not recommended it as a replacement for urine assessment. Herei...

The main objective of this study was to determine potential application of a serine proteinase derived from Asian pumpkin for obtaining biologically active peptides from casein. The course of casein hydrolysis by three doses of the enzyme (50, 150, 300 U/mg of protein) was monitored for 24 hours by the determinations of: hydrolysis degree DH (%), free amino group content (μmole Gly/g), RP HPLC ...