The dynamic range of protein expression in complex organisms coupled with the stochastic nature of discovery-driven tandem mass spectrometry (MS/MS) analysis continues to impede comprehensive sequence analysis and often provides only limited information for low-abundance proteins. High-performance fractionation of proteins or peptides prior to mass spectrometry analysis can mitigate these effec...

Sulpiride and tiapride are often used in the treatment of depression, schizophrenia and psychopathology of senescence, gastric or duodenal ulcers and are also partly excreted by kidney. This work developed a simple and sensitive method for their simultaneous monitoring in human urine based on capillary electrophoresis coupled with electrochemiluminescence detection by end-column mode. beta-Cycl...

BACKGROUND The present study aimed to demonstrate the seasonal activities of Ixodes ricinus at the pasture level and on the host. METHODS A vast pasture in Amol countryside (Mazandaran Province, Iran) which had the potential for a considerable number of cattle and sheep to graze was chosen. Tick sampling from the skin of 130 cattle and 130 sheep were collected every month interval. Simultaneo...

BACKGROUND The emergence of microfluidic immunosensors has provided a promising tool for improving clinical diagnoses. We developed an electrochemical immunoassay for the simultaneous detection of cardiac troponin I (cTnI) and C-reactive protein (CRP), based on microfluidic chips. METHODS The quantitative methodology was based on ELISA in poly(dimethylsiloxane)-gold nanoparticle composite mic...

The sites of action of angiotensin II along the nephron are not well defined and both proximal and distal effects are suggested. Using a microassay that permits measurement ofhormone binding in discrete tubule segments, we determined the binding sites of '25I-angiotensin II along the nephron of Sprague-Dawley rats. Specific binding in proximal convoluted tubule (PCT) (at 250C, pH 7.4) was linea...

We report here a unique approach to selectively label and detect specific RNA in an RNA mixture (without separation or purification) using DNA polymerase, dNTP labels, and a short synthetic DNA template complementary to the 3(')-terminus of the RNA. The detection sensitivity is high, at attomole level (10-18 mole). The selective principle was demonstrated by individually labeling and detecting ...

background: free-living amoebae have various genera that are found in several environmental niches such as soil, freshwater, dust, seawater and hot springs. most of free-living amoebae are normally harmless to humans. however, some such as acanthamoeba and also naegleria fowleri , balamuthia manderillaris and sappinia are identified as opportunistic and pathogenic amoebae that can cause eye dis...

Ultrasensitive molecular beacon (MB) DNA biosensors, with micrometer to submicrometer sizes, have been developed for DNA/RNA analysis. The fluorescence-based biosensors have been applied in DNA/ RNA detection without the need for a dye-labeled target molecule or an intercalation reagent in the testing solution. Molecular beacons are hairpin-shaped oligonucleotides that report the presence of sp...

BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is one of the aspects of metabolic syndrome (MetS). Due to the increase of MetS in Iran, this study was conducted to determine the prevalence of NAFLD, its potential predictors and their sex distribution in north of Iran, Amol. METHODS In 2008 this population based cross-sectional study included 5023 adult individuals who were randomly selec...