The Amplification Refractory Mutation System (ARMS) has been successfully applied to the detection of apolipoprotein (apo) E genotypes in human DNA extracted from peripheral blood. By using four allele-specific oligonucleotide primers and one common primer, one can identify the three common alleles of the apo E genetic polymorphism, epsilon 2, epsilon 3, and epsilon 4. The system amplifies two ...

We used the amplification refractory mutation system (ARMS)--a polymerase-chain-reaction-based method--to detect the 985 A-to-G mutation of the gene coding for the enzyme medium-chain acyl-CoA dehydrogenase (MCAD). The 985 A-to-G allele is thought to account for approximately 89% of the mutant alleles in this disorder and at least one copy was reported to be present in 98% of affected individua...

Objective(s):Organic anion transporter polypeptide 1B1 (OATP1B1) encoded by solute carrier organic transporter 1B1 (SLCO1B1) gene; a transporter involved in the uptake of drugs and endogenous compounds is present in hepatocyte sinusoidal membrane. Aim of this study was to investigate the frequencies of functionally significant SNPs (388A>G and 521T>C) and their haplotypes in 6 ethnic groups of ...

Backgrounds and Aims: P53 gene is regarded important in pathogenesis of different cancers. Therefore, this study aimed to investigate the frequency of p53 gene codon 72 Arg/Pro polymorphism in women suffering from breast cancer. Materials and Methods: A total of 90 patients with breast cancer and 83 matched healthy control women participated in this case-control study. Genomic DNA was extrac...

Background: Individuals respond to statins differently due to genetic variations. One of the most significant enzymes involved in drug metabolism is CYP2D6 enzyme, coded by the CYP2D6 gene. Individuals who carry two non-functional alleles in this gene are considered as poor metabolizers (PMs). Recognizing poor metabolizers might help in preventing adverse effects of drugs. Objective: In this ...

one of the leading causes of death in the world are cardiovascular diseases, among which coronary artery disease (cad) is the most common one. it occurs as a result of narrowing of the arteries which supply blood to the heart due to a theroma plaque formation. this kind of heart disease can be considered as a multifactorial one as genetic and environmental factors are involved in its incidence....

Introduction. Nephrin and podocin proteins, encoded by NPHS1 and NPHS2 genes, are essential for the integrity of the glomerular filter. The present study was aimed to investigate whether NPHS1 rs437168 and NPHS2 rs61747728 genetic variants are involved in the susceptibility to nephrotic syndrome (NS). Materials and Methods. This case-control study was performed on 108 children with NS and 97 he...