Some capsid proteins built on the ubiquitous HK97-fold have accessory domains imparting specific functions. Bacteriophage P22 coat protein has a unique insertion domain (I-domain). Two prior I-domain models from subnanometer cryoelectron microscopy (cryoEM) reconstructions differed substantially. Therefore, the I-domain's nuclear magnetic resonance structure was determined and also used to impr...

The ability of enzymes to harness free-radical chemistry allows for some of the most amazing transformations in nature, including reduction of ribonucleotides and carbon skeletal rearrangements. Enzyme cofactors involved in this chemistry can be large and complex, such as adenosylcobalamin (coenzyme B12), simpler such as “poor man’s B12” (S-adenosylmethionine and an iron-sulfur cluster), or ver...

background: the rodent somatosensory barrel cortex is an ideal model to examine the effect of experience-dependent plasticity on developing brain circuitry. sensory deprivation such as whisker deprivation may affect neuroanatomical aspects of the brain during developmental processes. the present study designed to investigate the possible effects of whisker deprivation on the morphometric charac...

Introduction: Barrel cortex of rats is a part of somatosensory cortex, which receives information from facial whiskers. Vibrisectomy by sensory deprivation leads to some changes in the barrel cortex, which have been known as experience dependent plasticity. On the other hand, Norepinephrine (NE) and locus coeruleus, which is the main source of NE, influenced response properties of cortical bar...

Bacterial autotransporters are comprised of an N-terminal 'passenger domain' and a C-terminal beta barrel ('beta domain') that facilitates transport of the passenger domain across the outer membrane. Following translocation, the passenger domains of some autotransporters are cleaved by an unknown mechanism. Here we show that the passenger domain of the Escherichia coli O157:H7 autotransporter E...

TonB-gated transporters have beta-barrels containing an amino-terminal globular domain that occludes the interior of the barrel. Mutations in the globular domain prevent transport of ligands across the outer membrane. Surprisingly, FepA with deletions of the globular domain (amino acids 3 to 150 and 17 to 150) was previously reported to retain significant sensitivity to colicins B and D and to ...

Heparanase is an endo-beta-d-glucuronidase capable of cleaving heparan sulfate, activity that is strongly implicated in cellular invasion associated with tumor metastasis, angiogenesis, and inflammation. In addition, heparanase was noted to exert biological functions apparently independent of its enzymatic activity, enhancing the phosphorylation of selected protein kinases and inducing gene tra...

introduction: in the present study the effect of nucleus basalis of meynert (nbm) destruction on the temporal characteristics of response integration evoked by multiple whisker stimulations in rat barrel cortex was studied. material and methods: wistar male rats weighing 250-350 g were used. the animals were randomly distributed into two groups: in the first group bilateral nbm lesions was indu...

Sentiment shifters, i.e., words and expressions that can affect text polarity, play an important role in opinion mining. However, the limited ability of current automated opinion mining systems to handle shifters represents a major challenge. The majority of existing approaches rely on a manual list of shifters; few attempts have been made to automatically identify shifters in text. Most of the...