In an attempt to localize topographic domains specific to native human chorionic gonadotropin (hCG), we studied the discontinuous antigenic regions recognized by a monoclonal anti-hCG antibody designated as C8 which binds only to hCG and does not cross-react with either the free hCG-alpha and hCG-beta subunits or other glycoprotein hormones. Using two-site monoclonal immunoradiometric assays (M...

Contents 1. Introduction 1 2. Residue and tube over cycle maps 2 3. Hodge structures on the complement 8 4. Period maps and their derivatives 11 5. Tautological deformations of hypersurfaces 12 6. The main theorem 13 Appendix A. Spectral sequences 16 A.1. Fundamental Notions 16 A.2. Hypercohomology 24 A.3. Abstract de Rham theorems 26 A.4. The spectral sequence of a filtered complex of sheaves ...

Postoperative adhesion can lead to an increase in the number of surgeries required, longer operation times, and high medical costs, resulting quality life patient being lowered. To address these clinical problems, we developed a surgical sealant with anti-adhesion properties for prevention postoperative following application large intestine surface. The was composed octyl (C8) group-modified Al...

The Chinese hamster cell mutant V-C8 is defective in the Brca2 gene (Kraakman-van der Zwet et al., 2002, Cell Biol.; 22: 669). Here we report that V-C8 cells were 10-fold more sensitive to camptothecin, an inhibitor of topoisomerase I, than the parental V79 cells. The level of the relaxation activity of topoisomerase I in nuclear extracts was also lower (4-fold) in V-C8 than V79 cells, in spite...

Triglycerides are the primary constituents of some seed kernels used in traditional Chinese medicine. Quality control of seed kernels containing multiple components with an environmentally friendly method is indispensable for establishing their quality standards (called monographs) in pharmacopeia. Using coix seeds (Semen Coicis) as an example, a green quantification strategy was proposed by co...

A challenge in the development of novel 18F-labelled positron emission tomography (PET) imaging probes is identification metabolically stable sites to incorporate 18F radioisotope. Metabolic loss from PET vivo can lead misleading biodistribution data as displaced accumulate various tissues.In this study we report on vitro hepatic microsomal metabolism caffeine containing bifunctional compounds ...

The purple phototrophic bacterium Rhodobacter sphaeroides utilises bacteriochlorophyll a for light harvesting and photochemistry. The synthesis of this photopigment includes the reduction of a vinyl group at the C8 position to an ethyl group, catalysed by a C8-vinyl reductase. An active form of this enzyme has not been identified in R. sphaeroides, but its genome contains two candidate ORFs (op...

Fluoroquinolones are antibacterial agents that attack DNA gyrase and topoisomerase IV on chromosomal DNA. The existence of two fluoroquinolone targets and stepwise accumulation of resistance suggested that new quinolones could be found that would require cells to obtain two topoisomerase mutations to display resistance. For wild-type cells to become resistant, the two mutations must be acquired...