The essential oils (EOs) and static headspaces (HSs) of in vitro plantlets and callus of Mentha x piperita were characterized by GC-MS analysis. Leaves were used as explants to induce in vitro plant material. The EO yields of the in vitro biomass were much lower (0.1% v/w) than those of the parent plants (2% v/w). Many typical mint volatiles were emitted by the in vitro production, but the call...

Eclipta alba is a medicinal herb, also known as Bhringraj belonging to family Asteraceae. The plant has hepatoprotective, antimicrobial and hair growth promoting properties. The effect of various phytohormones on in-vitro callus induction and shoot regeneration in Eclipta alba from leaf explant was evaluated. The sterilized explants were inoculated on MS medium supplemented with different conce...

Stevia rebaudiana Bertoni that conventionally propagated by seed or by cuttings or clump division which has a limitation of quality and quantity seed material. In present study, callus culture technique was tried to achieve rapid plant multiplication for quality seed material. Callus induction and multiplication medium was standardized from nodal as well as leaf sagments. It is possible to main...

Reciprocal differences persist in nature because of the unequal contribution of cytoplasmic determinants from male and female gametes to the zygote. The inheritance of genetic differences is an important factor that influences various traits, including somatic embryogenesis and regeneration in vitro. In this report, we estimate the cytoplasmic and maternal effects in pearl millet and their adeq...

Gloriosa superba L. a medicinally important monocot, member of the family Liliaceae, was investigated for callusing, direct regeneration and induction of hairy root using soil bacterium Agrobacterium rhizogenes. Friable callus was initiated from corms on basal MS medium amended with 2,4-D (1 mg L) and kinetin (1 mg L). Callus generated from such effective combinations were co-cultivated with th...

Calluses from seasonal mature shoots of Taxus globosa Schltdl. were subcultured in new medium containing 1 mg l -1 NAA, 0.5 mg l -1 2, 4-D, 0.025 mg l -1 BA. Calluses growing for 30 and 40 days in a medium added with 0.1-5.0 mg l -1 of chlorocholine chloride (CCC) were harvested for determination of taxol, phenolic compounds, and for polyphenol oxidase (PPO) activity. The results showed that by...

Leaf explants of kaladium dwubarwne Caladium bicolor (Aiton) Vent., were cultured in vitro on MS media supplemented with 25 different concentrations of BAP and NAA in order to determine the appropriate concentrations for micropropagation. All combinations induced callus formation on explants. Callus production on leaves explants grown on control medium was very low. The medium enriched with 4 m...

low efficiency of callus induction and green-plant regeneration have limited the application of anther culture in breeding programs. this study was conducted to determine the inheritance of callus induction and plant regeneration in rice (oryza sativa) anther culture. ten genotypes drived from a five-parent diallel mating set contain khazar, sepidrood, hashemi, alikazemi and nematt were evaluat...

to produce embryonic callus in four grapevine cultivars i.e. yaghouti, bidane sefid, shahroodi and flame seedless, ovary explants were collected at flower developmental stages iii and v and cultured in ms medium supplemented with various concentrations of 2,4-d and bap. for differentiation of somatic embryos, ms medium was supplemented with various concentrations of iaa and bap. results showed ...