The integrity of innermost layer the cornea, corneal endothelium, is key to sustaining transparency. Therefore, disease or injury causing loss damage endothelial cell population may threaten vision. Transplantation tissue standard treatment used replace malfunctioning cells. However, this surgery dependent upon donor tissue, which limited in supply. Hence, engineers have attempted construct alt...

PURPOSE To determine the extent to which CD95 ligand (CD95L) expressed on corneal epithelium and endothelium influences survival of cornea grafts placed orthotopically and heterotopically in the anterior chamber (AC), an immune-privileged site. METHODS Corneas from eyes of C57BL/6 (B6) and B6.gld (CD95L deficient) mice were (1) rendered into small full-thickness fragments, with or without an ...

PURPOSE To investigate the expression of cell-adhesion molecules on corneolimbal and neovascular endothelium and the associated leukocyte infiltration in an experimental model of inflammatory corneal neovascularization (NV). METHODS Corneal NV was induced in BALB/c mice by placement of nylon sutures. Interleukin-1 receptor antagonist (IL-1ra) was used topically to determine whether suppressio...

Human corneal endothelial cells have a limited ability to replicate in vivo and in vitro. Allograft transplantation becomes necessary when an accident or trauma results in excessive cell loss. The reconstruction of the cornea endothelium using autologous cell sources is a promising alternative option for therapeutic or in vitro drug testing applications. The native corneal endothelium rests on ...

Current treatment for corneal endothelial dysfunction consists in the replacement of endothelium by keratoplasty. Owing to scarcity donor corneas and increasing number transplants, alternative treatments such as cell-based therapies are necessary. In this article, we highlight biological aspects cornea endothelium, well context that surrounds need new alternatives conventional We then review so...