purpose: to evaluate the adverse effects of intravitreal injection of sterile chloramphenicol eye drops on corneal endothelial cells of donated corneas. methods: 100 donor corneas were enrolled in the study. one eye of each donor underwent intravitreal injection of sterile chloramphenicol eye drops following vitreous tap and the fellow eyes were served as controls. after corneo-scleral excision...

BACKGROUND The purpose of this study was to address the question of how the premature senescence process may affect corneal endothelium after penetrating keratoplasty, because the quality of donor corneal endothelial cells is important for corneal transplant success. METHODS The cell senescence and induced oxidative stress in corneal endothelium were assessed using a normal-risk orthotopic mi...

Corneal endothelium is a single layer of specialized cells that lines the posterior surface of cornea and maintains corneal hydration and corneal transparency essential for vision. Currently, transplantation is the only therapeutic option for diseases affecting the corneal endothelium. Transplantation of corneal endothelium, called endothelial keratoplasty, is widely used for corneal endothelia...

THE corneal endothelium appears to be a very interesting tissue from the morphological as well as physiological point of view. It forms a limiting layer separating the corneal stroma from the aqueous humour, and is therefore said by many authors to have a great physiological importance in the regulation of the water-balance of the corneal stroma. Thus it appears to be of foremost importance for...

THE corneal endothelium appears to be a very interesting tissue from the morphological as well as physiological point of view. It forms a limiting layer separating the corneal stroma from the aqueous humour, and is therefore said by many authors to have a great physiological importance in the regulation of the water-balance of the corneal stroma. Thus it appears to be of foremost importance for...

The major cause of corneal opacity and resultant visual loss is a critical decrease in corneal endothelial cell density. Due to the fact that corneal endothelial cells do not generally proliferate, cell densities gradually decrease in the corneal endothelium with age. Before we can begin to aid patients with decreasing endothelial cell densities, by inhibiting cell death or stimulating cell pro...

Corneal transparency is maintained by the corneal endothelium through its pump and barrier function. Severe corneal endothelial damage results in dysregulation of water flow and eventually causes corneal haziness and deterioration of visual function. In 2013, we initiated clinical research of cell-based therapy for treating corneal decompensation. In that study, we removed an 8-mm diameter sect...

BACKGROUND & OBJECTIVES Artificial corneal endothelium equivalents can not only be used as in vitro model for biomedical research including toxicological screening of drugs and investigation of pathological corneal endothelium conditions, but also as potential sources of grafts for corneal keratoplasty. This study was aimed to demonstrate the feasibility of constructing human corneal endotheliu...

Rabbit corneas denuded of their endothelium were coated with bovine corneal endothelial cells (from steers) previously maintained in tissue culture for short (20 generations) or prolonged (200 generations) periods. When grafted back into female rabbits, the corneal buttons remained clear and showed no edema. In contrast, denuded corneas coated with bovine keratocytes and grafted into rabbits be...