Background and purpose: We report the development of a very efficient cell-based high

Many genetically encoded biosensors use Förster resonance energy transfer (FRET) to dynamically report biomolecular activities. While pairs of cyan and yellow fluorescent proteins (FPs) are most commonly used as FRET partner fluorophores, respectively, green and red FPs offer distinct advantages for FRET, such as greater spectral separation, less phototoxicity, and lower autofluorescence. We pr...

Förster resonance energy transfer (FRET) microscopy is widely used to study protein interactions in living cells. Typically, spectral variants of the Green Fluorescent Protein (FPs) are incorporated into proteins expressed in cells, and FRET between donor and acceptor FPs is assayed. As appreciable FRET occurs only when donors and acceptors are within 10 nm of each other, the presence of FRET c...

We have studied the influence of the local density of optical states (LDOS) on the rate and efficiency of Förster resonance energy transfer (FRET) from a donor to an acceptor. The donors and acceptors are dye molecules that are separated by a short strand of double-stranded DNA. The LDOS is controlled by carefully positioning the FRET pairs near a mirror. We find that the energy transfer effici...

Background

ROBERT E. DEWAR, Western Ergonomics, Inc. DAVID FENNO, Texas Transportation Institute PHILIP M. GARVEY, Pennsylvania Transportation Institute BEVERLY T. KUHN, Texas Transportation Institute ARTHUR W. ROBERTS, New Jersey Department of Transportation FRANK SCHIEBER, University of South Dakota ALEX VINCENT, Transport Canada C. Y. DAVID YANG, H.W. Lochner, Inc. Y. B. YIM, University of California, ...

Washington : Government Printing Office. 1901. The collection of essays forming the general appendix of this annual report is always of most absorbing interest; and the volume before us, containing for example papers on Liquid Hydrogen, by Prof. Dewar; Psychical Research, by Sir W. Crookes; the Petrified Forests of Arizona, by Lester F. Ward, &c., &c., is if possible more fascinating than usual...

Resolve onboard the x-ray satellite X-Ray Imaging and Spectroscopy Mission (XRISM) is a cryogenic instrument with an microcalorimeter in Dewar. A lid partially transparent to x-rays (called gate valve or GV) installed at top of Dewar along optical axis. Because observations will be made through GV for first few months, transmission calibration crucial initial scientific outcomes. We present res...