two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (freesia ×hybrida bailey 'argenta'). interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. using 40% sodium hypochlorite solution for 40...

An efficient in vitro direct shoot regeneration system has been described for Lycium chinense Miller using leaf explants. Influence of various parameters such as growth regulator concentration, explant type, effect basal salt Murashige and Skoog (1962) medium (MS), Schenk Hildebrandt (1972) (SH), Gamborg et al. (1968) (B5), carbon sources (sucrose, maltose, fructose) on the regenerating shoots ...

Micropropagation of three genotypes of Indian mustard [Brassica juncea (L.) Czern.] using 7-days old seedling-derived transverse thin cell layer (tTCL) explants was accomplished. The genotype, explant source and addition of silver nitrate to the medium significantly influenced shoot bud induction. MS medium with 26.6 μM of 6-Benzylaminopurin (BAP) and 3.22 μM of 1-naphtaleneacetic acid (NAA) wa...

For efficient regeneration of Petunia × hybrida F1 'Opera Supreme Pink Morn', a simple in vitro micropropagation protocol was developed. Axillary bud explants dissected from 30-day-old in vitro-grown seedlings sprouted from hybrid seeds were cultured on Murashige and Skoog (MS) medium supplemented with 36 combinations of 6-benzylaminopurine (BA; 0.00, 0.50, 1.00, 2.00...

abstract lettuce (lactuca sativa l.) is a major leafy vegetable and belongs to the asteraceae family (compositae). the genetic manipulation of lettuce requires a reliable and efficient regeneration method. since lettuce is a suitable bioreactor for production of recombinant proteins especially edible vaccines. this study was conducted to identify an appropriate method for lettuce gene transform...

The present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (PGRs) combinations under in vitro conditions. The cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in MS medium containing BA (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and TDZ (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) e...

A method for tissue culture of micropropagation aquatic plant Staurogyne repens (Nees) Kuntze has been developed. Different phytohormones were investigated to optimize the growth explants. The shoot tip explants S. placed in MS nutrient medium including BAP (0.30-1.50 mg/l) + TDZ (0.10 NAA and Kn mg/l). Shoot regeneration frequencies changed as 77.77-100% 66.66-100% cultivation augmented with B...

Ridge gourd (Luffa acutangula L. Roxb) is a popular and nutritious vegetable in Bangladesh. The present investigation was undertaken to establish an efficient in vitro regeneration system of ridge gourd from immature embryo and cotyledon explants. Immature embryos and cotyledons were collected from immature seeds and culture on MS medium supplemented with various concentration and combination o...