In this study, we present a novel bead-incorporated centrifugal sample pretreatment microdevice to purify influenza A H3N2 viral RNA. Simple revolution per minute (RPM) control can lead to RNA capture on a bead-bed, and the sequential loading of a washing solution and an elution solution. Tetraethoxy orthosilicate (TEOS)-treated glass microbeads were utilized as a capture matrix. The sample pre...

AIM To develop a rapid real-time PCR method for the specific detection and quantification of Bacillus thuringiensis var. israelensis (Bti) spores present in the environment. METHODS AND RESULTS Seven soil samples as well as one sediment sample obtained from various regions of Switzerland and characterized by different granulometry, pH values, organic matter and carbonate content were artifici...

adenoviruses are recognized as common human pathogens that are responsible for a wide variety of infectious syndromes. bone marrow transplant patients are prone to life threatening opportunistic infections like adenoviruses. the nested polymerase chain reaction has provided an alternative, sensitive diagnostic method for detection of adenoviruses. in this study we developed pcr from hexon genes...

Article Type: Full Length Research Article Polymerase chain reaction (PCR) and loop mediated isothermal amplification (LAMP) represent a major advance in diagnostic methods in terms of speed and sensitivity. However, standard methods for sample preparation prior to PCR and LAMP are laborious and time consuming. Three template preparation methods (crude template, boiling, and commercial DNA extr...

Haplotypes, combinations of several phase-determined polymorphic markers, are extremely valuable for studies of disease association and chromosome evolution. Here we describe a technique called M1-PCR (M for "multiplex" and 1 for "single-copy DNA molecules") that enables direct molecular haplotyping of several polymorphic markers separated by as many as 24 kb. A genomic DNA sample first is dilu...

A procedure for the isolation of target PCR bands from multiplex PCR products using the E-Gel iBase Power System, an E-Gel Safe Imager Real-Time Transilluminator, and E-Gel CloneWell 0.8% SYBR Safe agarose cassettes is presented. The collected isolates are suitable for direct use in sequencing reactions without need of further purification. Reductions in time spent per sample and cost per seque...

background: human pathogens that can cause infertility may also affect sperm count and quality. viral infections can be considered as direct and/or indirect cause of male factor infertility. objective: our goal was to investigate the prevalence of herpes simplex virus in the semen of infertile men attending the avicenna infertility clinic, and to compare it with the herpes virus serology result...

Quantification of mRNA in single cells provides direct insight into how intercellular heterogeneity plays a role in disease progression and outcomes. Quantitative polymerase chain reaction (qPCR), the current gold standard for evaluating gene expression, is insufficient for providing absolute measurement of single-cell mRNA transcript abundance. Challenges include difficulties in handling small...

An efficientMonte Carlomethod for random sample generation fromhigh dimensional distributions of complex structures is developed. The method is based on random discretization of the sample space and direct inversion of the discretized cumulative distribution function. It requires only the knowledge of the target density function up to a multiplicative constant and applies to standard distributi...