conclusions direct sample pcr has the potential to rapidly and accurately diagnose candida vulvovaginitis in patients, especially if sufficient samples are obtained. results the results showed that out of the 150 samples, 55 were positive and 63 samples were negative by both methods, and 32 samples were positive using the culture method, but negative by ds-pcr. all positive ds-pcr samples had >...

Polymerase chain reaction (PCR) is commonly used for pathogen detection in clinical and environmental samples. These sample matrices often contain inhibitors of PCR, which is a primary reason for sample processing; however, the purification process is highly inefficient, becoming unacceptable at lower signature concentrations. One potential solution is direct PCR assessment without sample proce...

The novel HPV Direct Flow CHIP commercial system for Human Papillomavirus (HPV) genotyping is based on rapid PCR and automatic reverse dot blot hybridization to genotype-specific probes, allowing the detection of 36 HPV genotypes. This study examined the performance of HPV Direct Flow CHIP in formalin-fixed paraffin-embedded (FFPE) samples (n= 99). Each sample was analyzed both by Direct PCR, u...

With a few exceptions, the micro total analysis systems (mTASs) currently available have failed to live up to the ideal of the miniaturization of multiple laboratory operations onto a single chip. These systems perform sample preparation off chip and only pursue a single function. Hence, the definitive challenge is to interface the processing of real-world biological samples with downstream app...

The gut microbiome of animals is emerging as an important factor influencing ecological and evolutionary processes. A major bottleneck in obtaining microbiome data from large numbers of samples is the time-consuming laboratory procedures required, specifically the isolation of DNA and generation of amplicon libraries. Recently, direct PCR kits have been developed that circumvent conventional DN...

background and aims: lamivudine is amongst the antiviral for drug chronic hepatitis b treatment. during therapy with lamivudine, variants may emerge with ymdd mutation in the reverse transcriptase (rt) region of polymerase gene. this mutation might have a role in drug resistant for hbv. materials and methods: hbv dna extraction from serum sample of 88 patients, were subjected to nested pcr for ...