Nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) facilitates determination of both the kinetic constants (k(off)) and the equilibrium constants (K(d)) of complex dissociation from a single experiment. A typical NECEEM electropherogram consists of two peaks and an "exponential bridge" between them, smoothly merging into the peaks. The values of k(off) and K(d) are usuall...

Artemisinin and its derivatives are very important new class of antimalarial drugs. One of the most important artemisinin derivatives is artemether. The antiparasitic activity of artemether as a derivative of artemisinin is related to endoperoxide bridge in its structure. The aim of this study was the evaluation of antileishmanial effect of artemether, with more focus on its apoptotic effect. I...

Artemisinin and its derivatives are very important new class of antimalarial drugs. One of the most important artemisinin derivatives is artemether. The antiparasitic activity of artemether as a derivative of artemisinin is related to endoperoxide bridge in its structure. The aim of this study was the evaluation of antileishmanial effect of artemether, with more focus on its apoptotic effect. I...

Rpb5, a subunit shared by the three yeast RNA polymerases, combines a eukaryotic N-terminal module with a globular C-end conserved in all nonbacterial enzymes. Conditional and lethal mutants of the moderately conserved eukaryotic module showed that its large N-terminal helix and a short motif at the end of the module are critical in vivo. Lethal or conditional mutants of the C-terminal globe al...

Repair of DNA double-strand breaks in vertebrate cells occurs mainly by an end-joining process that often generates junctions with sequence homologies of a few nucleotides. Mre11 is critical for this mode of repair in budding yeast and has been implicated in the microhomology-based joining. Here, we show that Mre11 exonuclease activity is sensitive to the presence of heterologous DNA, and to th...

UNLABELLED Latent DNA replication of Kaposi's sarcoma-associated herpesvirus (KSHV) initiates at the terminal repeat (TR) element and requires trans-acting elements, both viral and cellular, such as ORCs, MCMs, and latency-associated nuclear antigen (LANA). However, how cellular proteins are recruited to the viral genome is not very clear. Here, we demonstrated that the host cellular protein, B...

in this paper, full scale load test of a horizontally curved steel box-girder bridge is carried out in order to detect structural defects, which reportedly result in unwanted vibrations in nearby buildings. the bridge is tested under the passage of six heavy vehicles at different speeds, so as to determine its static and dynamic responses. a total number of one hundred and two (102) sensors are...

dental implants have provided exceptional rehabilitative options for edentulous and partially edentulous patients. however, as more implants come into play, the more the clinicians come across problems where specific considerations must be taken into account to meet expectations. the toronto bridge is a treatment modality proposed for restoring several teeth lost in patients with increased crow...

this study redescribes an arthroscopic bridge technique for repair of avulsion of the posterior cruciate ligament. the procedure is performed step-to-step. in this technique we created two bone tunnels in the anterior aspect of the tibia and inferior medial of the tibia tuberosity and then create suture fixation of the fragment using the suture bridge. the arthroscopic bridge technique was used...