The receptor binding domain of the tailspike protein Gp9 from the P22 bacteriophage was recently shown to reduce Salmonella colonization in the chicken gut. In this study, we transiently expressed the receptor binding domain of the Gp9 tailspike protein in Nicotiana benthamiana, and targeted it to the endoplasmic reticulum (ER) or to the chloroplasts. Gp9 was also fused to either an elastin-lik...

A facile technique was demonstrated for the controlled assembly and alignment of multi-segment nanowires using bioengineered polypeptides. An elastin-like-polypeptide (ELP)-based biopolymer consisting of a hexahistine cluster at each end (His(6)-ELP-His(6)) was generated and purified by taking advantage of the reversible phase transition property of ELP. The affinity between the His(6) domain o...

In this work, a proof of concept elastin-like polypeptide-Z domain fusion (ELP-Z) based affinity precipitation process is developed for monoclonal antibody (mAb) purification from industrial harvest feeds. Greater than 99% mAb recoveries are obtained during the initial binding step of the process for both pure mAb and the mAb harvest feeds. Great than 90% overall mAb yields are also obtained fo...

Formulas For Intraocular lens Power calculatIon Modern cataract surgery replaces the natural lens of the eye with an intraocular lens (IOL). After surgery, the optical system of the eye will consist of two major refractive elements, the cornea and the newly inserted IOL. This two-lens system is at the basis of IOL power calculation. To facilitate calculations, both the cornea and the IOL are co...

Treatment of glioblastoma is complicated by the tumors' high resistance to chemotherapy, poor penetration of drugs across the blood brain barrier, and damaging effects of chemotherapy and radiation to normal neural tissue. To overcome these limitations, a thermally responsive polypeptide was developed for targeted delivery of therapeutic peptides to brain tumors using focused hyperthermia. The ...

Defining impact significance is the main technical task that influences decision-making during Environmental Licensing Procedure (ELP). The ELP begins with screening to determine potentially significant impacts of proposed project. Scoping then follows address any interventions deemed worthy attention in production an Impact Assessment (EIA). This will include consideration relevant landforms a...

The production of pharmaceutical proteins in plants has made much progress in recent years with the development of transient expression systems, transplastomic technology and humanizing glycosylation patterns in plants. However, the first therapeutic proteins approved for administration to humans and animals were made in plant cell suspensions for reasons of containment, rapid scale-up and lack...

Eicosanoids may play a role in ischemic stroke. However, the associations of variants in cyclooxygenase (COX) pathway genes and interaction among these variants with carotid plaque vulnerability are not fully understood. In present study, twelve variants in COX pathway genes were examined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry method in 396 patients w...

The delivery of anticancer therapeutics to solid tumors remains a critical problem in the treatment of cancer. This study reports a new methodology to target a temperature-responsive macromolecular drug carrier, an elastin-like polypeptide (ELP) to solid tumors. Using a dorsal skin fold window chamber model and intravital laser scanning confocal microscopy, we show that the ELP forms micron-siz...

We consider issues of stability of time-discretization schemes with exact treatment of the linear part (ELP schemes) for solving nonlinear PDEs. A distinctive feature of ELP schemes is the exact evaluation of the contribution of the linear term, that is if the nonlinear term of the equation is zero, then the scheme reduces to the evaluation of the exponential function of the operator representi...