PURPOSE To evaluate the validity of a keratometry (K)-independent method of estimating effective lens position (ELP) before phacoemulsification cataract surgery. SETTING Institute of Eye Surgery, Whitfield Clinic, Waterford, Ireland. DESIGN Evaluation of diagnostic test or technology. METHODS The anterior chamber diameter and corneal height in eyes scheduled for cataract surgery were meas...

The aim of this study was to evaluate the effects of eucalyptus leaf powder (ELP) and eucalyptus essential oil (EEO) on growth performance and immune response of broiler chickens. A total of 160 broiler chicks were assigned randomly into five dietary treatments from 7-42 days of age. Dietary treatments included: a control diet, control diets plus 1,000 or 3,000 mg/kg of ELP, and control diets p...

Invasive plants can inflict great harm, yet drivers of successful invasion remain unclear. Many invaders of North American deciduous forests exhibit extended leaf phenology (ELP), or longer growing season relative to natives. ELP may grant invaders competitive advantages, but we argue that ELP more potently drives invasion in the presence of herbivores. ELP invaders can support herbivores by le...

Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease digestion. Therefore, an additional chromatographic purification step is required to remove the proteases, and this...

Through genetic engineering technology, catalase (CAT) and superoxide dismutase (SOD) have been separately fused to an elastin-like polypeptide (ELP). Thus, the enzymes can be purified through phase transition. Hexadecylamine-modified poly(aspartic acid) (HPASP) is able to self-assemble, forming soft microparticles. The HPASP microparticles were used to co-immobilize SOD-ELP and CAT-ELP through...

Elastin-like polypeptides (ELPs) composed of a VPGXG repeat undergo a reversible phase transition in aqueous solution. They are hydrophilic and soluble in aqueous solution below their transition temperature (T(t)), but they become hydrophobic and aggregate when the temperature is raised above their T(t). In this study, we examine the quantitative uptake of a fluorescence-labeled, thermally resp...

The limited throughput, scalability and high cost of protein purification by chromatography provide motivation for the development of non-chromatographic protein purification technologies that are cheaper and easier to implement in a high-throughput format for proteomics applications and to scale up for industrial bioprocessing. We have shown that genetic fusion of a recombinant protein to an e...

D-amino acid oxidase (DAAO) and catalase (CAT) have been genetically modified by fusing them to an elastin-like polypeptide (ELP). ELP-DAAO and ELP-CAT have been separately immobilized on multi-walled carbon nanotubes (MWNTs). It has been found that the secondary structures of the enzymes have been preserved. ELP-DAAO catalyzed the oxidative deamination of D-alanine, and H2O2 was evolved contin...

PURPOSE To test the reliability and interobserver variation of external lacrimal punctum (ELP) grading. METHODS In a population-based epidemiologic study, ELP was visually graded based on slit lamp examination (grade 0 to 5), as described previously. Grades 0, 1, and 2 are for closed or stenotic ELP and grade 3 is for normal ELP (subjective grades). Grades 4 and 5 are for larger than normal (...

An edge cut C of a graph G is tight if |C∩M|=1 for every perfect matching M G. Barrier cuts and 2-separation are called ELP-cuts, which two important types in covered graphs. Edmonds, Lovász Pulleyblank proved that has nontrivial cut, then it also ELP-cut. Carvalho, Lucchesi, Murty made stronger conjecture: given any G, there exists ELP-cut D does not cross C. We confirm the conjecture this pap...