Continuous production of nisin in laboratory media and whey permeate was investigated using a packed-bed bioreactor. Lactococcus lactis subsp. lactis ATCC 11454 was immobilized by natural attachment to fibre surfaces and entrapment in the void volume within spiral wound fibrous matrix. The inoculated bioreactor was fed M17 medium and effect of pH, temperature, dilution rate, and substrate conce...

the application of neural networks to model a laboratory scale inverse fluidized bed reactor has been studied. a radial basis function neural network has been successfully employed for the modeling of the inverse fluidized bed reactor. in the proposed model, the trained neural network represents the kinetics of biological decomposition of organic matters in the reactor. the neural network has b...

A pilot-scale sand-based fluidized bed bioreactor (FBBR) was utilized to treat both methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA) from a contaminated aquifer. To evaluate the potential for re-use of the treated water, we tested for a panel of water quality indicator microorganisms and potential waterborne pathogens including total coliforms, Escherichia coli, Salmonella and Shigel...

A pilot scale membrane bioreactor hybrid system (MBR-HS) was evaluated for municipal wastewater treatment. This novel system comprised of a granular activated carbon-sponge fluidized bed bioreactor (GACS-FBBR) followed by a submerge membrane bioreactor (MBR) with the capacity of 2 L/min. The results indicated that the MBR-HS could effectively remove 90% DOC and 95% NH₄-N. PO₄-P removal efficien...

Transient and continuous recombinant protein expression by HEK cells was evaluated in a perfused monolithic bioreactor. Highly porous synthetic cryogel scaffolds (10 ml bed volume) were characterised by scanning electron microscopy and tested as cell substrates. Efficient seeding was achieved (94% inoculum retained, with 91-95% viability). Metabolite monitoring indicated continuous cell growth,...

A mathematical model was developed and tested to simulate the generation and transfer of heat in solid substrate fermentation (SSF). The experimental studies were realized in a 1-L static bioreactor packed with cassava wet meal and inoculated with Aspergillus niger. A simplified pseudohomogeneous monodimensional dynamic model was used for the energy balance. Kinetic equations taking into accoun...

A unique biotechnological method for remediation of industrial wastewater contaminated by toxic mercury, based on the enzymatic reduction of ionic mercury by live bacteria, has been developed by prof. Deckwer and co-workers at GBF (at present HZI), Germany, and implemented in a pilot-plant scale. The experience gained during operation of this installation led to the idea, that the process of bi...