Magnetic resonance microimaging was used to measure diffusion decay over an extended b-factor range in a formalin-fixed normal prostate sample and a Gleason pattern 3+4 cancer tissue sample. The coefficients of biexponential fits to diffusion decay data from 1600 voxels of dimension 160 × 160 × 160 μm(3) in each sample were correlated with underlying epithelial and stromal compartment partial v...

The Golgi method can be successfully applied to human embryonic and foetal nervous tissues which have been fixed in formol for years provided the scalp and skull of the specimen were opened originally for proper fixation. The following method is a variation of the Golgi method which has proved successful: 1 Wash the complete embryo or foetus (up to the age of 12 weeks-crown-rump length 87 mm) i...

Identification and quantitation of candidate biomarker proteins in large numbers of individual tissues is required to validate specific proteins, or panels of proteins, for clinical use as diagnostic, prognostic, toxicological, or therapeutic markers. Mass spectrometry (MS) provides an exciting analytical methodology for this purpose. Liquid Tissue MS protein preparation allows researchers to u...

Title of dissertation: PROTEOME ANALYSIS OF FORMALIN-FIXED AND PARAFFINEMBEDDED TISSUE Tong Guo, Doctor of Philosophy, 2008 Dissertation directed by: Professor Cheng S. Lee Department of Chemistry and Biochemistry Because of the long history of the use of formalin as the standard fixative for tissue processing in histopathology, these archival formalin-fixed and paraffinembedded (FFPE) tissues ...

Diffusion tensor microimaging at 16.4 T with 40µm isotropic voxels was to investigate anisotropic water diffusion in prostate tissue at spatial resolution approaching the cellular scale. Nine normal tissue and two cancer samples were collected from the peripheral zone of six formalin fixed radical prostatectomy specimens. Fibromuscular stromal tissue exhibited microscopic diffusion anisotropy (...

The development of prognostic and diagnostic biomarkers, such as those from gene expression studies, requires independent validation in clinical specimens. Immunohistochemical analysis on tissue microarrays (TMAs) of formalin-fixed paraffin-embedded tissue is often used to increase the statistical power, and it is used more often than in situ hybridization, which can be technically limiting. He...

In process of fabrication of a fixed partial denture, dies are trimmed to expose margins of the preparations. The need for the soft tissue cast is quite evident as the soft tissue emergence profile that surrounds the prepared tooth is destroyed in the process of fabrication. This article describes a modified technique to fabricate the soft tissue cast for the conventional fixed partial denture....