avian intestinal spirochaetosis (ais) is a condition arising from colonization of the caeca and colon of birds with anaerobic spirochaetes of the genus brchyspira. the purpose of the present study was to evaluate pcr amplification from formalin-fixed, paraffin-embedded tissue method for detecting infected laying hens with brachyspira pilosicoli  with the same primers that have been used for det...

Background Sample preparation is the first step in the evaluation of tissue samples. In this research tried to using two types of formalin and Bouin fixative ovarian tissue morphogenesis be evaluated practical. MaterialsAndMethods Ovaries of 6-8 week-old Naval Medical Research Institute female mice were randomly divided into 3 groups: Ι. Ovaries that immediately fixed in Bouin’s fixative; II. O...

purpose: to determine the dissection level during deep lamellar keratoplasty (dalk) using the big-bubble technique by light microscopy (lm) and transmission electron microscopy (tem). methods: descemet’s membrane (dm) and stromal tissue of 4 keratoconic eyes were removed because of inadvertent intraoperative dm perforation during dalk which was converted to penetrating keratoplasty. these speci...

fixed myocardial defects in both stress and rest images, could be artifactual as a result of soft tissue attenuation. to increase specificity and identify the false positive results, we used gated technique to evaluate the wall motion and wall thickening as an index to differentiate real ischemic lesions from artifactual defects. 93 patients were studied. in 46 patients (48.8%) fixed perfusion ...