The amounts of 27 amino acids and other ninhydrin-reacting substances were determined by ion-exchange chromatography in the urine of patients with psoriasis, eating diets containing 54 and 104 Gm. of protein from the same source. Regardless of level of protein intake, asparagine plus glutamine, histidine, glycine, and taurine or lysine contents of urine accounted for between 55 and 64 per cent ...

Three series of nitrogen-balance experiments were carried out on growing rats fed on purified isonitrogenous diets (16 g N/kg) to study the importance of non-essential N and the essential:total N (E:T) ratio for attaining maximum N balance (NB) and biological value (BV) of protein. Minimum dietary levels of asparagine, proline and glutamic acid required for maximum NB and BV were estimated to b...

In order to study the effects of various levels of protein in diet based on total and digestible amino acids on performance and carcass traits of broilers, an experiment was conducted as a completely randomized design with 288 broiler chicks from Cobb 500 strain in 6 treatments, each treatment consist of 3 replicates with 16 chicks per replicate. Treatments were the diets as follow: 1) diet for...

A study was conducted in a completely randomized design to evaluate the effect of diets formulated on the basis of four critical essential amino acids (lysine, methionine, tryptophan and threonine) on the crude protein requirement, carcass quality, nutrient digestibility, haematological and blood biochemical indices of broiler finisher chickens (28-56 days) reared under tropical environment. On...

We studied the levels of twenty two free amino acids in the striatum and olfactory bulb of mice treated during nine weeks with daily intraperitoneal injections of manganese chloride at a concentration of 5.0 mg Mn+2/kg body weight. In the olfactory bulb the contents of alanine, alpha-amino-n-butyrate, arginine, asparagine, aspartate, citrulline, GABA, glutamate, glycine, isoleucine, leucine, me...

In the present study, we identified free amino acid (FAA) contents and chemical composition in four populations of Salmo trutta macrostigma living in Mediterranean region of Turkey. In addition, taste impacts of FAAs were evaluated by taste active values. Moisture, protein, fat and ash content were found in the ranges of 75.49 - 79.59 %, 16.94 - 19.97 %, 1.58 - 3.75 % and, 1.39 - 1.56 %, respec...

Deamidation is a major fragmentation channel upon activation by collision induced dissociation (CID) for protonated peptides containing glutamine (Gln) and asparagine (Asn) residues. Here, we investigate these NH3-loss reactions for four Asn- and Gln-containing protonated peptides in terms of the resulting product ion structures using infrared ion spectroscopy with the free electron laser FELIX...

L-Asparagine uptake by Stemphylium botryosum is mediated by two distinct energy- and temperature-dependent transport systems. One permease is relatively specific for L-asparagine and L-glutamine and is present in nutrient-sufficient mycelium. The specific permease shows an optimum pH at 5.2, saturation kinetics (Km = 4.4 x 10(-4) M, Vmax = 1.1 mumol/g per min), competitive gradient of L-asparag...

The minimum requirements for eliciting or enhancing ornithine decarboxylase activity (EC. 4.1.1.17); L-ornithine carboxylase) in neuroblastoma cells incubated in salts-glucose solutions have been investigated. These incubation conditions permit the study of changes in ornithine decarboxylase activity independently of the growth-associated reactions that occur in cell culture media (Chen, K.Y. a...

Preimplantation mouse embryos exhibit growth at the blastocyst stage when there is a dramatic increase in protein content between days 4 and 5 after hCG treatment. This increse in protein synthesis requires the uptake of amino acids from the surrounding medium, but the consumption of individual amino acids present as a mixture has not been examined. We therefore measured the simultaneous deplet...