We propose a mean-field theory of chemical and physical planar brushes of linear gradient copolymers swollen in a selective solvent. The polymer chains are grafted to the substrate by the ends with the excess of insoluble monomer units, and the majority of the soluble units are located near the free ends of the chains. The grafting points are considered to be immobile (chemical brush) and mobil...

Grain fermentability largely determines the feed value of grains for ruminants. Our objective was to evaluate the variation in kinetics of gas production of cereal grains and the relationship among gas production, chemical composition and feed value. Eighteen barley, 99 corn, 23 sorghum, and 57 wheat samples were fermented in vitro for 48 h. Gas production was measured with a computerized syste...

Using fluorescent antibody staining, we have established the association of methionyl-tRNA synthetase with the endoplasmic reticulum in PtK2 cells. After Triton X-100 extraction, 70% of the recovered aminoacyl-tRNA synthetase activity was found in the detergent-insoluble fraction. This fraction of the enzyme remained localized with insoluble endoplasmic reticulum antigens and with ribosomes, wh...

Single chain Fragment variable (scFv) is an antibody fragment consisting variable regions of heavy and light chains. scFvs enhance their penetrability into tissues while maintaining specific affinity and having low immunogenicity. Insoluble inclusion bodies are formed when scFvs are expressed in reducing bacterial cytoplasm. One strategy for obtaining functionally active scFv is to translocate ...

Species from the genus Bacillus have the ability to form endospores, dormant cellular forms that are able to survive heat and acid preservation techniques commonly used in the food industry. Resistance characteristics of spores towards various environmental stresses are in part attributed to their coat proteins. Previously, 70 proteins have been assigned to the spore coat of Bacillus subtilis u...

IN a series of papers [Chibnall and Channon, 1927, 1, 2, 3; Channon and Chibnall, 1927], we have recorded certain results which we have obtained in the analysis of the ether extract of the leaf-cell cytoplasm of the cabbage. The object of the present paper is to discuss these results so far as is at present possible from their chemical and physiological aspects, and, in passing, to refer to our...

two experiments were conducted to evaluate the crude protein fractionation, in vitro gas production and the biological effects of tannins in alfalfa silage treated with different levels of pistachio by-products (pb) extract. pb was soaked in water with a ratio of 1:5 (w/v) for 12 h. after filtering and concentrating, the crude extract was added to fresh alfalfa to a final concentration of 1%, 2...