I N RECENT YEARS, specffic inborn erythrocyte enzyme deficiencies have l)een implicated in the etiology of certain hereditary hemolytic anemias not characterized by spherocytosis. This report defines still another instance in which hereditary hemolytic anemia has been found to be associated with a deficiency in a specific glycolytic enzyme. The propositus has had a hemolytic syndrome present si...

OBJECTIVE Patients with diabetes mellitus have an increased risk of suffering atherothrombotic syndromes and are prone to clustering cardiovascular risk factors. However, despite their dysregulated glucose metabolism, intensive glycemic control has proven insufficient to reduce thrombotic complications. Therefore, we aimed to elucidate the determinants of thrombosis in a model of type 2 diabete...

The functions of individual amino acid residues in the active site of Thermoanaerobacterium thermosulfurigenes D-xylose ketol-isomerase (EC 5.3.1.5) were studied by site-directed substitution. The role of aromatic residues in the active-site pocket was not limited to the creation of a hydrophobic environment. For example, Trp-188 provided for substrate binding and Trp-139 allowed for the discri...

Fructose is a versatile precursor for food and chemicals. Currently, catalytic production of fructose achieved by enzymatic isomerization glucose from renewable lignocellulose. Although the catalyst, isomerase, selective, it not stable. Here, aluminum-containing metal-organic frameworks (Al-MOFs) are shown to be active, stable, reusable in ethanol. Al-MOFs 64% selectivity with 82% conversion at...

Giardia intestinalis encystment results in the incorporation of galactosamine (GalN) (a cystwall specific sugar) into outer cyst wall filaments [1,2]. GalN is synthesized during encystment from endogenous glucose by an inducible enzyme pathway [3] and the first reaction unique to GalN synthesis is the conversion of fructose-6phosphate (F6P) to glucosamine 6-phosphate (G1cN6P) [4,5]. In G. intes...

Phosphoglucose isomerase (D-glucose 6-phosphate ketol isomerase, EC 5.3.1.9) of the human erythrocyte was resolved into a major and two minor components. The major enzyme form, isomerase a, was purified to constant specific activity and appeared homogeneous bjl chromatography, electrophoresis, and ultracentrifugation. The minor enzyme forms, isomerases b and c, were also prepared in high purity...

The xylA gene coding for xylose isomerase from the hyperthermophile Thermotoga neapolitana 5068 was cloned, sequenced, and expressed in Escherichia coli. The gene encoded a polypeptide of 444 residues with a calculated molecular weight of 50,892. The native enzyme was a homotetramer with a molecular weight of 200,000. This xylose isomerase was a member of the family II enzymes (these differ fro...