results in vitro analysis showed that the greater the concentration of the extract, the greater the cytotoxicity. conclusions the crude extract of p. australis might be cytotoxic at high concentrations as evaluated against the cell line. materials and methods the mtt cytotoxicity assay was performed on a 3t3 mouse fibroblast cell line to measure the in vitro cytotoxic activity of the crude form...

BACKGROUND Wound healing is a complex and dynamic process following damage in tissue structures. Due to extensive skin damage caused by burn injuries, this study determined the role of human adult peripheral and umbilical cord blood platelet-rich plasma on proliferation and migration in human skin fibroblasts. METHODS Platelet-rich plasma (5, 10, 15, 20 and 50% PRP) from human umbilica...

background: prostate cancer is one of the leading causes of cancer deaths among men. new gene expressed in prostate (ngep), is a prostate-specific gene expressed only in normal prostate and prostate cancer tissue. because of its selective expression in prostate cancer cell surface, ngep is a potential immunotherapeutic target. to target the ngep in prostate cancer, it is essential to investigat...

context connective tissue cells include fibroblasts, chondrocytes, adipocyte, and osteocytes. these cells are specialized for the secretion of collagenous extracellular matrix and are responsible for the architectural framework of the human body. evidence acquisition connective tissue cells play a central role in supporting as well as repairing tissues and organs. fibroblast cell therapy could ...

The progression of a normal cell to senescence in vivo and in vitro is accompanied by a reduction in the length of the telomeres, the chromosome capping segments at the end of each linkage group. However, overexpression of the reverse transcriptase subunit (HTERT) of the ribonucleoprotein telomerase restores telomere length and delays cellular senescence. Although some data exist in the literat...

Objective: This study aimed to evaluate the cytotoxic effects of Arctium minus (Hill) Bernh. ssp. methanol extract derived from aerial parts on cancer cell lines.Material and Method: For cytotoxicity assays, two different human breast lines (MCF-7 MDA-MB-231) healthy fibroblast line (MRC-5)as a control were used. Cell viability determination was performed using CellTiter-Blue method. One-Way AN...

A new series of 1,2-diaryl-4,5,6,7-tetrahydro-1H-benzo[d]imidazoles, possessing trimethoxyphenyl pharmacophore, were synthesized to evaluate their biological activities as tubulin inhibitors. Cytotoxic activity of the synthesized compounds 7a-f was assessed against several human cancer cell lines, including MCF-7 (breast cancer cell), HEPG2 (liver hepatocellular cells), A549 (adenocarcinomic hu...

Background and purpose: Cancer is one of the major health challenges in the world. The efficacy of current treatments is low but their side effects are high. Cold atmospheric plasma (CAP) is a new modality for cancer treatment. This study aimed to compare the cytotoxicity effect of CAP on the cell line models of common cancers and normal cells. Materials and methods: In this experimental study...

shiga toxins (stxs) are bacterial virulence factors produced by shigella dysenteriae serotype 1 and escherichia coli strains. stxs are critical factors for the development of diseases such as severe bloody diarrhea and hemolytic uremic syndrome. additionally, stxs trigger the secretion of pro- inflammatory cytokines and chemokines, particularly in monocytes or macrophages. the inflammatory cyto...