results cloning and subcloning of the flic gene were confirmed by colony pcr, restriction enzymes digestion and dna sequencing of the recombinant plasmids pprime-flic and pvax-flic. the expression of flagellin protein in eukaryotic cells was approved by immunofluorescence assay (ifa), western blotting analysis and the reverse transcriptase polymerase chain reaction (rt-pcr) method. conclusions t...

mesenchymal stem cells (mscs),as the major stem cells for cell therapy, have been studied from different aspects in pre-clinical and clinical settings for more than a decade. these cells modulate the immune system (humoral and cellular immune responses) in vitro by producing soluble factors (anti-inflammatory molecules) and/or making cell-cell contacts. hence they could be used in regenerative ...

alterations have been found to occur in every component of immune response during an anesthesia and surgery. these alterations represent the body's general physiological responses and are mainly depended on the extent of surery as well as on blood transfusion. basically, the immune response to an anesthesia and surgery is a beneficial reaction needed in local defences, wound healing, and p...

fully mature dendritic cells (dcs) play pivotal role in inducing immune responses and converting naïve t lymphocytes into functional th1 cells. we aimed to evaluate listeria monocytogenes-derived protein fractions to induce dc maturation and stimulating t helper (th)1 immune responses. in the present study, we fractionated listeria monocytogenes-derived proteins by adding of ammonium sulfate in...

Zataria multiflora essential oil possesses immunomodulatory effects and the present study investigatesits effect on immune responses to live Newcastle disease (ND) vaccines and faecal shedding period ofvaccine strain virus in chickens. One hundred and eighty chickens from both sexes were divided into 6 equalgroups: group 1: unvaccinated, group 2: vaccinated (B1 vaccine followed by La Sota 10 da...

This study was conducted to evaluate the effects of dietary inclusion of thyme essential oil (TEO) on growth performance and immune responses of broiler chicks subjected to heat stress (HS). Broilers were divided into five groups; group 1) chicks fed with basal diet and reared under thermoneutral condition; group 2) chicks fed with basal diet and subjected to HS condition; and groups 3, 4 and 5...

Cancer immunotherapy has gained a lot of interest over the past few years due to the success of immune checkpoint inhibitors in treating cancer (1, 2). Immune checkpoint inhibitors, such as monoclonal antibodies against cytotoxic T-lymphocyte–associated antigen 4 (CTLA-4) and programmed death 1 (PD-1), have been shown to increase survival of patients with advanced cancers (1, 2). These in...

In induction of systemic and mucosal immunity, particulate antigens are more effective than soluble antigens possibly because they are more efficiently endocytosed by mucosal-associated lymphoid tissue (MALT) M cells. In this study, we determined the systemic and mucosal immune responses in rabbits following intranasal immunization of tetanus toxoid TT and CpG-ODN encapsulated within PLGA nanos...