The use of nitrogen in nutrient media is essential for growth and helps identify highly productive media for potato (Solanum tuberosum L.) micropropagation and adventitious shoot formation. Three potato cultivars (Agria, Marfona and Savalan cultivars) were examined for their growth response with medium containing four different levels of KNO3 (1, 1.25, 1.5 and 1.75 times as much 1.9 g l-1) and ...

Introduction Pinus taeda stands for productivity and quality of its timber [1]. Researches using biotechnology are of great importance and have been applied to the improvement of its timber and plantation [2]. The main method of Pinus propagation is by seeds, once the minicuttings depends on the season of the year or depends of juvenile material [3-5]. Thus, researches on micropropagation of Pi...

Development of a micropropagation system for a new species or cultivar can be a daunting task and hundreds of decisions are involved in even deciding where to start. Dozens of basal media, growth regulators, and supplemental nutrients are available for use. Environmental conditions to be considered include light intensity and quality, photoperiod, temperature, container, amount of medium, liqui...

An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole acetic acid (IAA) and 50 mg/l adenine sulphate as RBC design experiment. Two approaches were taken to reduce the chemical cost ...

Plant biotechnology has emerged as a powerful tool of crop improvement and has aroused a great deal of interest in many countries because of its application to agriculture and horticulture. We report here the application of a number of in vitro plant breeding techniques such as micropropagation, in vitro flowering, in vitro pollination and fertilisation, anther culture, somatic embryogenesis, a...

In order to optimize the micropropagation of Qare-Qat (Vaccinium arctostaphylus J.J.Sm), samples were collected from two regions of Iran (Asalem and Kelardasht). Anderson (AN) media containing different concentrations of zeatin (0, 0.5, 1, 2 and 4 mg L-1) and different levels of pH (4.5, 5 and 5.5) were compared. The in vitro shoots were transferred on AN media supplemented with zeatin (1 and 2...

Plant growth regulators (PGRs) have an important role in callus induction and organogenesis of plant explants cultured in vitro conditions. Callus has a proper potential for plant regeneration through indirect organogenesis and embryogenesis as well suspension culture, genetic transformation and production of secondary metabolites. In current experiment, leaf explants of Petunia × hybrida F1 we...

An efficient protocol is suggested for in vitro culture of five Phalaenopsis hybrids obtained by hand-cross-pollination of three commercial hybrids Calgary, Ankara, and Kendall. Four nutrient media- namely half-strength Murashige and Skoog, Knudson, Phytamax containing activated charcoal, and Mitra– once supplemented (with coconut water, peptone, or both), and once without any supplement were c...

One of the most effective ways to propagate Lilium is by using tissue culture techniques, preferably through bulblet production. In addition to the surviving percentage of Lilium bulbs after transplantation, the small size of in vitro bulblets compared to commercial bulbs and bulblets‟ dormancy are the most important constraints to commercializing Lilium micropropagation. Different concentratio...