The wheat leaf rust is widespread throughout Ukraine, which makes it necessary to monitor the effectiveness of host plant resistance genes. In 2019–2020, under conditions subpopulation pathogen, typical for Right Bank Forest-Steppe zone known genes was determined. Evaluation conducted after 2018 epiphytotypic series on a nearly isogenic Thatcher lines and cultivars that expand core gene set. Ac...

Rett syndrome (RTT) is an autism spectrum developmental disorder caused by mutations in the X-linked methyl-CpG binding protein 2 (MECP2) gene. Excellent RTT mouse models have been created to study the disease mechanisms, leading to many important findings with potential therapeutic implications. These include the identification of many MeCP2 target genes, better understanding of the neurobiolo...

A permanently high virulence was found in tachyzoites of T. gondii K24 after serial passage in mice (90 passages during 324 days). Virulence tests revealed that a single tachyzoite of the 50th passage represented LD100 for mice. Analysis of genotype of K24 isolate was done by PCR/RFLP with ROP1/Ddel, SAG1/Ddel, 850/Rsal and IGS/Rsal and by RFLP/DNA with TGR1E sequence and Pstl enzyme. K24 isola...

Genetic heterogeneity though common in tumors has been rarely documented in cell lines. To examine how often B-lymphoma cell lines are comprised of subclones, we performed immunoglobulin (IG) heavy chain hypermutation analysis. Revealing that subclones are not rare in B-cell lymphoma cell lines, 6/49 IG hypermutated cell lines (12%) consisted of subclones with individual IG mutations. Subclones...

Hillerød, Denmark) by agglutination with latex beads and type-specific serum and by microscopic determination of capsule swelling with type-specific serum (SSI Diagnostika, Hillerød, Denmark), according to the manufacturer’s instructions. These methods gave concordant results. Etests (bioMérieux, Solna, Sweden) demonstrated that the isolate was sensitive to all antimicrobial drugs tested; MIC w...

The disease mechanism of Rett syndrome (RTT) is not well understood. Studies in RTT mouse models have suggested a non-cell-autonomous role for astrocytes in RTT pathogenesis. However, it is not clear whether this is also true for human RTT astrocytes. To establish an in vitro human RTT model, we previously generated isogenic induced pluripotent stem cell (iPSC) lines from several RTT patients c...

Staphylococcus aureus (S.aureus) is an important zoonotic pathogen implemented in various hospital, community as well livestock infections. Pets dogs and cats have increased their close social relation with human leading to significant elevation transmission of zoonoticmultidrug resistant virulent pathogens.Diplex polymerase chain reaction was applied on ten clinical isolates obtained from woun...

Durability of plant disease resistance (R) genes may be predicted if the cost of pathogen adaptation to overcome resistance is understood. Adaptation of the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo), to virulence in rice is the result of the loss of pathogen avirulence gene function, but little is known about its effect on aggressiveness under field conditions. We evaluated...

abstract ascochyta blight caused by didymella rabiei is one of the most important diseases of chickpea in ilam and kermanshah provinces, iran. in order to determine the pathogenicity variation of didymella rabiei populations, infected plants in field crops of different regions of both provinces were sampled. one hundred isolates were isolated by chickpea seed meal dextrose agar media. these iso...