PURPOSE The purpose of this study was to identify phenotypic markers of human limbal stem cells in fetal and adult corneas. METHODS RNA from microscopically dissected superficial limbal and central fetal (18 weeks) corneas was amplified and used to generate P(32)-labeled, reverse-transcribed antisense RNA that was linearly amplified and hybridized to a focused stem cell cDNA microarray. Diffe...

PURPOSE Human Wharton's jelly stem cells (HWJSCs) are able to differentiate into skin and oral mucosa epithelial-like cells. In this work, we demonstrate for the first time the capability of HWJSCs to differentiate in vitro into cornea epithelial-like cells in a three-dimensional model. METHODS First, primary cell cultures of HWJSCs, corneal epithelial cells, and corneal keratocytes were cult...

A patient with chemical corneal burn presented two months after the acute episode of chemical injury to his right eye (OD) and was diagnosed with severe limbal stem cell deficiency and with vascularized corneal opacity OD. Limbal cell transplantation and penetrating keratoplasty (PKP) was performed. The autologous and allograft limbal tissue included peripheral cornea, limbus and conjunctiva ob...

Protective coat. This, the outermost coat, consists of the transparent cornea and the opaque sclera; their curvatures are different, and the region of junction is called the limbus or limbal region. The cornea is a living structure, containing cellscorneal corpuscles-and being lined by epithelium and endothelium, so that its nutrition is a matter of concern for us at this meeting. By contrast, ...

Fluoresceinated IgG was injected centrally into the rabbit's cornea and both its rate of loss from the tissue and its rate of spread in the stroma were estimated by objective fluorometry. A new technique for determining the rate of loss was established which required only the measurement of the maximum fluorescence in the tissue. The rate at which freshly formed IgG in the blood would arrive at...

IT is still not clear how the cornea which has no vessels in its tissue is nourished, but the problem of the nutrition of tissue is closely related to the vascular structure, so that if we wish to solve the problemi of corneal nutrition we must examiine the constructioni and functionis in niormal anid abnormal situations of the finest conjunctival capillaries aIt the limbtis terminating in the ...

This paper outlines changes to the ocular surface caused by contact lenses and their degree of clinical significance. Substantial research development improve oxygen permeability rigid soft has meant that in many countries issues hypoxia have largely been negated. The ability change axial growth characteristics globe is being utilised help reduce myopia pandemic several studies meta-analyses sh...

BACKGROUND The giant filtering bleb encroaching onto the corneal surface is a rare occurrence in our and other's clinical experience (Kapoor and Syed, Int. Ophthalmol 31(5):403-404, 2011), even in patients having had a trabeculectomy with mitomycin C, and how it developed is debated. In this paper, we report a patient who developed a huge overhanging filtering bleb after trabeculectomy, and pre...

With this investigation, the anatomy of bulbus oculi was studied in 12 Akkaraman sheep. The oculi’s specific morphological features breed were introduced study. In border cornea and scle-ra, limbus ended at point where it entered under sclera. eyes examined, existence rima cornealis sclera not observed. Based on observations made, center thinner com-parison with its periphery. sheep, determined...