stem cells located at the limbus ,  are the ultimate source for regeneration of the corneal epithelium in the normal and traumatized states.  when limbal stem cells are dysfunctional or deficient, limbal stem cell deficiency (lscd) develops. its surgical management depends on laterality and severity of corneal-limbal involvement. conventional methods of stem cell transplantation are conjunctiva...

Background  To report normal variations of the limbal structures using in vivo laser scanning confocal microscopy. Methods: This was a retrospective study of fourteen eyes from 11 healthy individuals. Confocal imaging of cornea and limbus was performed using Heidelberg Retina Tomograph III Rostock Corneal Module. Results: The typical structure of the palisades of Vogt (POV) was detected ...

PURPOSE We investigated the expression of laminin-5 and integrins, and the ultrastructure of the interface between basal cells and the basement membrane in the keratoconus cornea. These findings were compared to those in normal central cornea and limbus. METHODS Frozen sections of the normal cornea (center and limbus) and the keratoconus cornea were immunostained with monoclonal antibodies ag...

PURPOSE To quantify the orientation of the collagen fibrils in the human limbus and to compare it with the orientation in the cornea and the sclera. METHODS Fibril orientation was measured from 100 synchrotron x-ray diffraction patterns collected at intervals along lines across the cornea, limbus, and sclera. RESULTS A distinct circumcorneal annulus of collagen fibrils was revealed in the l...

BACKGROUND Identification of stem cells from a corneal epithelial cell population by specific molecular markers has been investigated previously. Expressions of P63, ABCG2 and K14/K5 have all been linked to mammalian corneal epithelial stem cells. Here we report on the limitations of K14/K5 as a limbal stem cell marker. METHODOLOGY/PRINCIPAL FINDINGS K14/K5 expression was measured by immunohi...

PURPOSE To evaluate the use of neoepitope monoclonal antibody BKS-1, which recognizes keratanase-generated keratan sulfate (KS) stubs on keratan sulfate proteoglycans in human cornea, limbus, and sclera. METHODS BKS-1 specifically recognizes a keratanase-generated neoepitope [N-acetyl-glucosamine-6-sulfate (GlcNAc-6-S)] at the nonreducing terminal of corneal and skeletal KS glycosaminoglycan ...

In our previous studies, we noted a non-uniform distribution of protein tracer preferentially entering the anterior stromal lamellae of the cornea from the limbus. Given other differences reported previously between the anterior and posterior lamellae of the cornea, and the number of corneal disorders in which abnormalities are preferentially confined to either the anterior or posterior lamella...

background  to report normal variations of the limbal structures using in vivo laser scanning confocal microscopy. methods: this was a retrospective study of fourteen eyes from 11 healthy individuals. confocal imaging of cornea and limbus was performed using heidelberg retina tomograph iii rostock corneal module. results: the typical structure of the palisades of vogt (pov) was detected in 57% ...

There was a partial thickness laceration of the right cornea at the junction of the middle and lower thirds extending in an irregular horizontal line from side to side. From the depth of this laceration the cornea was split to the lower limbus and in the lamellar pocket so formed were embedded multiple fragments of a shattered corneal contact lens. At no point did this lamellar laceration penet...

eyf served as a control. Intraperitoneal [Ii ]-thyeidine was injected. Three eyes were studied histologically and autoradiographically at 1 hr, 3 hr. 6 hr. 9 hr, 12 hr, 24 hr, 2 d, 3 d, 9 d, 12 d, 15 d, 18 d and 21 d. The epithelial cells were scanned from limbus to limbus. Analysis of variance and linear regression (SPSS package) were used for statistical evaluation. Results: The cells prolife...