introduction: an induction of osteogenic differentiation in rat bone marrow stromal cell cultures by dexamethasone was reported. materials and methods: the marrow stromal cells obtained from 4 to 6 weeks - old spruge-dawely male rats were grown in primary culture for 7 days and then subcultured for 20 days. the cells were cultured in either dmem medium containing 15% fetal calf serum, antibioti...

Gap junctions (GJs) provide for a unique system of intercellular communication (IC) allowing rapid transport of small molecules from cell to cell. GJs are formed by a large family of proteins named connexins (Cxs). Cx43 has been considered as the predominantly expressed Cx by hematopoietic-supporting stroma. To investigate the role of the Cx family in hemopoiesis, we analyzed the expression of ...

Two human stromal cell lines were established previously from bone marrow-derived primary long-term cultures by immortalization using the SV40 large T antigen and cellular cloning. After irradiation, the fibroblast-like cell lines L87/4 and L88/5 support hematopoietic differentiation of allogeneic cord blood cells in vitro. The stromal cells do not express CD34 and CD50, but some adhesion molec...

This study was performed to investigate the expression of reactive oxygen species (ROS)-related proteins and to analyze the implications for primary and metastatic breast cancer. We constructed a tissue microarray containing 143 metastatic breast cancers (52 lung metastases, 38 bone metastases, 37 brain metastases, and 16 liver metastases) and performed immunohistochemical staining for ROS-rela...

The aorta-gonads-mesonephros (AGM) region autonomously generates the first adult repopulating hematopoietic stem cells (HSCs) in the mouse embryo. HSC activity is initially localized to the dorsal aorta and mesenchyme (AM) and vitelline and umbilical arteries. Thereafter, HSC activity is found in the urogenital ridges (UGs), yolk sac, and liver. As increasing numbers of HSCs are generated, it i...

gastrointestinal stromal tumors are mesenchymal neoplasms of the gastrointesti- nal tract that originate from all areas of the gastrointestinal tract. metastases to the liver, peritoneum, bones, lungs and soft tissues have been reported. we present the case of a 47- year-old woman with rectal gastrointestinal stromal tumor that underwent wide local excision. she was treated with imatinib for a ...

Extracellular vesicles (EVs) have been recently reported as crucial mediators in cell-to-cell communication in development and disease. In this study, we investigate whether mesenchymal stromal cells that constitute a supportive microenvironment for hematopoietic stem and progenitor cells (HSPCs) released EVs that could affect the gene expression and function of HSPCs. By taking advantage of tw...

Abstract Aim Hepatocellular carcinoma (HCC) is a primary liver cancer that usually develops in background of chronic disease and prolonged inflammation. A major contributor the complex molecular pathogenesis HCC highly intertwined cross-talk between tumor surrounding stromal cells, such as hepatic stellate endothelial macrophages other immune cells. These tumor-stroma interactions actively fuel...