Rat liver was homogenized in isotonic buffer, fractionated by differential centrifugation, and then subfractionated by equilibrium sedimentation in Nycodenz gradients. Fractions were assayed for both Cu,Zn-SOD and Mn-SOD by exploiting the cyanide-sensitivity of the former activity and by the use of specific antibodies. As expected, the cytosol and lysosomal fractions contained Cu,Zn-SOD; while ...

Due to rapid industrialization and urbanization, environmental pollution has become a significant concern in developing countries; therefore, the main objective of the current study is to determine some of the heavy metal (HM) concentrations in the leaves of dusty miller (Cineraria maritima) and purstane (Mesembryanthemum cordifolium) in different general bus terminal along with the green space...

the study aimed to come up with a list of specific macroalgae species, which could be used to biomonitor specific metal elements in the coastal waters of east africa. water extraction, edta, aqua regia extraction and optimized bcr 3-step sequential extracts were used to mimic bioavailable metals under various environmental conditions. the results indicated that ulva lactuca could be used as a b...

This study compares the effectiveness of multi-micronutrient formulations containing iron (Fe), manganese (Mn), and zinc (Zn) with traditional (EDTA, DTPA, HEEDTA, and EDDHAm) or novel chelates (o,p-EDDHA, S,S-EDDS, and IDHA) and natural complexing agents (gluconate and lignosulfonate). The stability and reactivity of the formulations were studied on batch experiments with calcareous soil and b...

Superoxide dismutases' (SODs) expression is altered in several diseases including Alzheimer, atherosclerosis, cancer and psoriasis. Previously, we reported a marked increase in Mn-SOD and Cu,Zn-SOD functional activity in human dermal psoriatic fibroblasts. As retinoic acid (RA) has been used in the treatment of psoriasis and a mechanism for its beneficial effects is not understood, we investiga...

Alcohol damage to the liver can, among other factors, be mediated through the action of toxic oxygen radicals generated by ethanol. Major antioxidants in the liver are copper/zinc and manganese superoxide dismutases (Cu/Zn- and Mn-SODs). In order to test whether SODs may be differentially expressed in alcoholic liver disease (ALD), biopsies from 45 patients with ALD were analyzed for qualitativ...

STREET R., SZÁKOVÁ J., DRÁBEK O., MLÁDKOVÁ L. (2006): The status of micronutrients (Cu, Fe, Mn, Zn) in tea and tea infusions in selected samples imported to the Czech Republic. Czech J. Food Sci., 24: 62–71. A total of 30 tea samples of different origins, thirteen green tea samples, thirteen black tea samples, two semi-fermented tea samples and one white tea, imported to the Czech Republic, wer...