This study investigated the association between both polymorphisms and metabolic marker changes of leptin gene, body weight, weight gain, carcass traits, feed intake, conversion ratio. Blood samples were collected from 60 V-line Baladi Black rabbits for DNA extraction biochemical analysis. sequencing (202-bp) revealed four non-synonymous single nucleotide polymorphism (SNPs) that characterized ...

Background: Mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene can cause congenital bilateral absence of vas deferens. Yet, the spectrum and frequency of CFTR mutations in Indian males with congenital absence of vas deferens (CAVD) is unknown. Materials and Methods: We investigated 50 Indian males, diagnosed with unilateral or bilateral absence of vas deferens at t...

Growth hormone (GH) is the most important hormone that controls somatic cell growth and syntheses of protein, fat, and carbohydrates. This study was conducted to identify gene polymorphism of GH-1 using PCR -single strand conformation polymorphism (SSCP) technique and its association with growth traits including condition factor (CF), body weight (BW) and total length (TL) in common carp (Cypr...

the aims of the present study were to propose apolymerase chain reaction-restriction fragment length polymorphismpcr-rflp genotyping method for the ay_376689:c.773c>t single nucleotide polymorphism snp of the equine prkag3 gene, related to muscle performance, as well as to characterize this snp and a second polymorphism, aawr_02017454:g.121684t>c of the spata1 gene, related to male fertility, i...

Simple sequence repeats (SSRs) markers are important molecular tools widely used in genetic and genomic research plants. However, the number of SSR developed lentils (Lens culinaris Medik.) so far, which is an economically cool season legume plant, has been very limited. The lack among main factors limiting lentil breeding studies. In this study, 288 clones belonging to enriched libraries with ...

the green fluorescent protein (gfp) was originally isolated from the jellyfish aequorea victoria that fluoresces green when exposed to blue light. gfp protein is composed of 238 amino acids with the molecular mass of 26.9 kd. the gfp gene is frequently used in cellular and molecular biology as a reporter gene. to date, many bacterial, yeast , fungal, plants, fly and mammalian cells, including h...

avian influenza is a contagious viral disease affecting the respiratory, digestive and nervous system of birds belong to orthomyxovirus family. in the chicken mx gene, type of amino acid at position 631 determines the antiviral activity against influenza and vesicular stomatitis virus. a single nucleotide polymorphism (snp) at position 2032, responsible for the variation of amino acid at positi...

Background & Aims: SLC26A4 gene mutations are the second identifiable genetic cause of autosomal recessive nonsyndromic hearing loss (ARNSHL) after GJB2 mutations and are currently investigated in molecular diagnosis.In databases, several potential STR markers related to this region have been introduced. In this investigation, the characteristics and informativeness of D7S2425 CA repeat STR mar...

Cajal-Retzius (CR) cells are early-developing cells important in mammalian corticogenesis. Reelin, a protein secreted by CR cells, is essential for completion of neuronal migration and cortical lamination. Lack of reelin causes the 'reeler' phenotype in mice and autosomal recessive lissencephaly with cerebellar hypoplasia in man. Focal increases in reelin and CR cells are associated with thicke...