A high resolution ultrasound imaging technique, ultrasound backscatter microscopy (UBM), has previously been shown to be useful for in utero imaging of mouse embryos, and for direct manipulation of mouse embryos through UBM-guided injections. UBM images from mouse embryos staged between 8.5 and 10.5 days of gestation are presented to demonstrate the range of anatomical structures which can be s...

background: nowadays, oocytes and embryos vitrification has become a routine technique. based on clinical judgment, re-vitrification maybe required. but little is known about re-vitrification impact on genes expression. objective: the impact of re-vitrification on apoptotic and implanting genes, bax, bcl-2 and erbb4, at compaction stage embryos were evaluated in this study. materials and method...

Karl Oskar Illmensee studied the cloning [2] and reproduction of fruit flies, mice, and humans [3] in the US and Europe during the twentieth and twenty-first centuries. Illmensee used nuclear transfer techniques (cloning [2]) to create early mouse [4] embryos from adult mouse [4] cells, a technique biologists used in later decades to help explain how embryonic cells function during development....

Background: Diabetes is a metabolic disease caused by a deficiency in the pancreatic secretion of insulin and/or by the inability of tissues to efficiently respond to insulin, events that cause hyperglycemia and affect all organs.Tissue alterations caused by diabetes affect different organic systems , including the male reproductive system. Reproductive dysfunction is one of the common secondar...

l(2)dtl (lethal (2) denticleless), is an embryonic lethal homozygous mutation initially identified in Drosophila melanogaster that produces embryos that lack ventral denticle belts. In addition to nucleotide sequence, bioinformatic analysis has revealed a conservation of critical functional motifs among the human L2DTL, mouse L2dtl, and Drosophila l(2)dtl proteins. The function of the L2DTL pro...

Mitochondria are cellular organelles regulating metabolism and cell death pathways. This study examined changes in mitochondrial membrane potential (deltapsim) throughout the stages of preimplantation development in mouse embryos conceived either in vivo or in vitro and human embryos donated to research from IVF. Embryos stained with the deltapsim-sensitive dye (JC-1) were quantified for the ra...

In mice, a minimum number of healthy embryos is required to trigger and maintain pregnancy. Therefore, when recovering mouse embryos from a limited litter, one useful technique is to transfer carrier ICR embryos along with the embryos of interest, a technique referred to as cotransfer. In this study, we examined suitable mouse strains for cotransfer with C57BL/6J (B6) embryos in regards to the ...

Criteria for assessing embryo vitality of denuded rabbit and mouse embryos in vitro were: volume of the embryo, number of cells and number of metaphase plates after 24 and 48 h in culture. Mouse embryos grew normally, with and without the zona pellucida, but denuded rabbit embryos showed reduced growth (volume and cell number) and failure to implant in synchronized recipients.