Background: Several methods for sex selection of embryos have been used in research centers and infertility centers till now. Materials and Methods: In this research, the effect of medium, by placing the mouse (NMRI) spermatozoa in the medium containing certain concentrations of albumin and ascorbic acid, separation of spermatozoa containing X and Y chromosomes and doing in vitro fertilization ...

Background: ROS are free radicals that have a significant role in many of the sperm physiological processes. In the presence of polyunsaturated fatty acids such as sperm plasma membrane,ROS triggers a chain of chemical reactions called lipid peroxidation.The aim of this study was to determine and compare the concentration of MDA and NO in testicular extract of hyperprolactinemic adult male mice...

Among the numerous anti-sperm monoclonal antibodies generated in our laboratory, HS-33 was shown to react with a conserved antigen on the acrosome of spermatozoa from human and mouse. By using indirect immunofluorescent assay, it was demonstrated that HS-33 did not bind to live human sperm. However, this antibody was found to react with the methanol-fixed acrosome-intact, but not with acrosome-...

To initiate fertilization, mouse sperm bind to Ser- (O-) linked oligosaccharides located at the sperm combining site of zona pellucida glycoprotein mZP3. Apparently, the oligosaccharides are present on one or more of five Ser residues clustered in the carboxyl-terminal region of the mZP3 polypeptide. Here, each of the Ser residues, as well as an intervening Asn residue, was converted to a small...

objectives: the aim of the present study was to evaluate protective effect of royal jelly on sperm parameters, testosterone level, and malondialdehyde (mda) production in mice. materials and methods: thirty-two adult male nmri mice weighing 30±2 g were used. all the animals were divided into 4 groups. control group: received saline 0.1 ml/mouse/day orally for 30 days. royal jelly group (rj): re...

Sperm capacitation is a maturation event that takes place in the female reproductive tract and is essential for fertilization. A family of phospholipid-binding proteins present in bovine seminal plasma (BSP proteins) binds the sperm membrane at ejaculation and promotes bovine sperm capacitation. Homologues of these proteins have also been isolated from boar, ram, goat, bison and stallion semina...

To fertilize eggs, mammalian sperm must first undergo a form of cellular exocytosis called the acrosome reaction. In 1983, my laboratory reported that nanomolar concentrations of ZP3, one of three zona pellucida glycoproteins from mouse eggs, induced mouse sperm to undergo the acrosome reaction in vitro. Seven years later, this article from Florman’s laboratory (O’Toole et al., 2000) revealed t...

The genotoxicity of the ethanolic extract betel nut was evaluated using sarcoma 180 tumour bearing mouse considering sperm motility, viability, biochemical estimation fructose in seminal fluid and head morphology assays. Sperm studied by H-E staining Toluidine blue method. But method is a reliable to evaluate DNA damage sperms. Ethanolic BNE (betel extract) can suppress percentage viability lev...

TAS1R taste receptors and their associated heterotrimeric G protein gustducin are involved in sugar and amino acid sensing in taste cells and in the gastrointestinal tract. They are also strongly expressed in testis and sperm, but their functions in these tissues were previously unknown. Using mouse models, we show that the genetic absence of both TAS1R3, a component of sweet and amino acid tas...