One of the methods for treating oncological diseases is application short-lived radionuclides. Existing risks intoxication and radioactive contamination can be eliminated by introduction a radon isotope into hard strong fullerene coat. To increase effectiveness these drugs it promising to introduce structural fragments amino acids, peptides, steroid hormones, nucleic bases or heterocyclic compo...

The genus Streptomyces is known as producers of bioactive compounds that have antibacterial and antioxidant properties. sp. SAE4034 was to produce can inhibit kill pathogenic bacteria, including Staphylococcus aureus, but the group their inhibitory mechanisms were not yet known. ability in producing antioxidants unknown yet. This research aimed determine classification produced by SAE4034, know...

conclusions consequently, small diameter scnt interaction with nucleic acid bases is noncovalent. also, the results revealed that small diameter scnt interaction especially scnt (4, 4) with nucleic acid bases can be useful in practical application area of biomedical fields such detection and drug delivery. objectives hence, the quantum mechanics (qm) method based on ab initio was used for this ...

The mechanism by which drugs that target nucleic acids cause neurotoxicity is not well described. We characterized the neurotoxicity of Hoechst 33342 (bis-benzimide), a common cell permeable nuclear dye, in primary neuronal cultures. The mechanism of cell death was not apoptotic, as death is rapid, not accompanied by typical nuclear morphological changes, and is insensitive to inhibitors of tra...

INTRODUCTION The use of aptamers, antisense technology and RNA interference has allowed nucleic acids to be considered as promising alternatives to classical drugs. However, nucleic acids face several obstacles in the creation of effective nucleic acid drugs. The development of these approaches has strengthened the pipeline with an increasing number of these therapies in clinical trials. AREA...

A simple method for the detection of sequence- and structural-selective ligand binding to nucleic acids is described. The method is based on the commonly used thermal denaturation method in which ligand binding is registered as an elevation in the nucleic acid melting temperature (T(m)). The method can be extended to yield a new, higher -throughput, assay by the simple expediency of melting des...

Catalytic nucleic acids are regarded as potential therapeutic agents and biosensors. The catalytic activities of nucleic acid enzymes are usually investigated in dilute aqueous solutions, although the physical properties of the reaction environment inside living cells and that in the area proximal to the surface of biosensors in which they operate are quite different from those of pure water. T...

nine labeled either with C'4 or N'5. Considerable controversy has developed as to which one of these tracer compounds gives an accurate measurement of the “relativeturnover-rate― of the nucleic acids. In order to determine the “absolute turnover rate― of the nucleic acids, it is necessary to know the specific activity of the immediate precursor of the nucleic acids. Since even the ident...

background & objectives: antisense peptide nucleic acids (pna) that target growth essential genes show potent bactericidal properties without cell lysis. we considered the possibility that whether pna treatment influence the bacteria total nucleic acids content and apply approach to develop a new delivery system to dendritic cells (dcs). dcs are the most potent antigen presenting cells in t...

As one of the initial steps towards the determi nation of the metabolism and mechanism of action of fluorinated pyrimidines, 5-fluorouracil and 5fluoroorotic acid were labeled with radiocarbon in the 2-carbon atom. We have previously reported briefly that fluorouracil is incorporated, as such, into the combined nucleic acids of mouse liver, spleen, and Ehrlich ascites cells (9). The present rep...