The incorporation in vivo of compounds labeled with N15 or Cl4 into the purines and pyrimidines of nucleic acid has been repeatedly demonstrated (e.g. (l-3)). W e wish to report observations on the incorporation of radioactive erotic acid in vitro into the pyrimidines of the nucleic acid of rat liver slices. It is believed that this procedure will readily permit the study of precursors of nucle...

Nucleic acids are biologically active materials, and chemically modified nucleic acids are now being used in nucleic acid drugs. DNA, one of the two types of nucleic acids, can also be used as a material to construct DNA-based delivery systems, such as DNA hydrogel, for therapeutic compounds. Use of chemically modified nucleic acids would greatly increase the therapeutic potency of such DNA-bas...

Lividomycin inhibited incorporation of acetate-114C , glutamic acid-1-14C, leucine-1-14C, and glycine1-14C into protein of Mycobacterium smegmatis (strain Jucho). Incorporation of acetate-1-14C and glycine1-14C into nucleic acids was also inhibited, but incorporation of these compounds into lipids was not inhibited in the presence of lividomycin. Incorporation of uridine-2-14C into nucleic acid...

We developed a site-specific method for precipitating inorganic compounds using organic compounds, DNA, and designed peptides with peptide nucleic acids (PNAs). Such a system for site-specific precipitation represents a powerful tool for use in nanobiochemistry and materials chemistry.

We describe the synthesis of three angelicin derivatives which can be used for labeling nucleic acids with biotin. These compounds were used to label nucleic acids in the presence of lysed cell constituents. The resulting labelled nucleic acids show hybridization to a genus specific probe for E. coli. The relative comparison of sensitivity indicates that a polyamine linker is better than a poly...

The idea of using nucleic acid molecules as therapeutic agents was conceived in the 1970s with the development of antisense strategies. Antisense compounds are single-stranded nucleic acids that, in principle, disrupt the synthesis of a targeted protein by hybridizing in a sequence-dependent manner to the mRNAs that encode it. The mechanism of inhibition by nucleic acid aptamers is fundamentall...

The major groups of biochemical compounds (proteins, lipids, carbohydrates, chitin, nucleic acids and nucleotides) have a distinct functional role and, therefore, incorporation of biochemical interpretations into stoichiometric analysis would improve our current understanding of species life histories and their interactions. A review of the stoichiometric composition of the main biochemical com...

This survey focuses on recent developments in the radiation chemistry of purine bases in nucleic acids and related model compounds. Both direct and indirect effects of ionizing radiation are investigated with special emphasis on the structural characterization of the final decomposition products of nucleic acid components. Available assays for monitoring radiation-induced base lesions are criti...

Endosomal TLRs play an important role in innate immune response as well as in autoimmune processes. In the therapy of systemic lupus erythematosus, antimalarial drugs chloroquine, hydroxychloroquine, and quinacrine have been used for a long time. Their suppression of endosomal TLR activation has been attributed to the inhibition of endosomal acidification, which is a prerequisite for the activa...

Nucleic acid extracted from environmental samples is an important analyte in instrumental analysis. With respect to the phenomenon which sodium tungstate promotes methane production anaerobic digestion, analyses of microbial community using RNA were conducted; however, we noticed that only concentration measured by absorbance measurement (NanoDrop) was remarkably high. Therefore, this study, DN...