background:class ii transactivator (ciita) is a dominanttranscriptional element, controlling numerous genes in theimmune system. ciita is expressed in a constitutive pattern inantigen presenting cells although its expression can occur inother cell types. since the revelation of ciita, there have beenconsiderable advances toward understanding its role as anactivator of mhc ii genes in humans and...

Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...

background: mycobacterium tuberculosis is the causative agent of tuberculosis (tb). bacille calmette-guerin (bcg) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult tb vaccine. the aim of this study was to develop a new tuberculosis dna vaccine candidate encoding a recombinant hspx-ppe44-esxv fusion antigen of m. tuberculosis. methods: a fu...

conclusions in this experimental study, for the first time in iran the expression and purification of this recombinant protein was done successfully. this recombinant protein could be used as a vaccine candidate and diagnostic purpose in subsequent investigations. results the highest expression of the cytochrome p450 cyp141 protein was obtained by the addition of 1 mm of isopropyl β-d-1-thiogal...

introduction: gene expression and purification of luciferases from the firefly, lampyris turkestanicus, and optimization of cellular atp measurements were performed. methods: cdna encoding luciferases from lampyris turkestanicus was transferred from pqe30 vector into pet28a expression vector and pltu28 was built. newly constructed vector was expressed in e. coli xl1 blue and the recombinant luc...

the main purpose in this paper, to express strong algorithmic optimization in their ability to detect expression target of sar that use in aircraft, plane and satellites to observe the target on the ground. for this purpose,the sar technology and its applications are introduced. previous algorithms used in this field with poor diagnosis and appropriate speed is low, like means and fuzzy and pso...

conclusions this study indicated that the optimization of gene transfer increases the expression of igf-1. high concentrations of igf-1, in combination with cell therapy, have a significant effect on wound healing. results in vitro, the maximum expression of igf1 (96.95 pg/ml) in transfected fibroblast cells was 24 hours after gene transfer. in vivo, it was clear that igf-1 gene therapy caused ...

background:the premature termination of high producer clones, which will be killed due to cell proliferation and proteins production antagonism, is one of the basic drawback in recombinant proteins technology. furtheremore, it is supposed some toxic proteins like interferon which we intended to clone and express, inhibit host cells’ proliferation. so, it is necessary to tightly control ifn-γ pr...