BACKGROUND The present study aims to investigate the effect of noscapine (0.5-2.5 μM), an alkaloid from the opium poppy, on primary murine fetal cortical neurons exposed to oxygen-glucose deprivation (OGD), an in vitro model of ischemia. METHODS Cells were transferred to glucose-free DMEM and were exposed to hypoxia in a small anaerobic chamber. Cell viability and nitric oxide production were...

Due to poor tumour-associated vasculature, tumour cells are subjected to a fluctuating microenvironment with periods of limited oxygen and glucose availability. Adaptive mechanisms to adverse microenvironments are important for tumour cell survival. The cyclooxygenase (COX)-2/prostaglandin E(2) (PGE(2)) pathway has key roles in colorectal tumorigenesis. Although glucose is important as an energ...

Introduction: The Serum/Glucose deprivation -induced cell death in cultured PC12 cells represents a useful in vitro model for the study of brain ischemia and neurodegenerative disorders. Nigella sativa L. has been known as a source of antioxidants. To elucidate the neuroprotective actions of N. sativa extract in vitro, we studied the effect of N. sativa extract on cultured PC12 cells under s...

The present study evaluates the effect of grifolin (GFL) in oxygen/glucose deprivation (OGD) induced white matter lesion. Injury induced with OGD was found to be significant at the 9th h of OGD induction and the effect of GFL on the proliferation of oligodendrocyte precursor cells (OPCs) was assessed by CCK-8 and Hoechst 33258 assay at GFL 1, 5, 25, 50 and 100 μm concentrations. Whereas immunoc...

Brain ischemia is one common cerebrovascular disease with unfavorable prognosis and higher morbidity and mortality rates. The re-perfusion injury after primary ischemia further aggravated the progression of cerebral ischemia. The development of effective drugs for protecting neurons during brain ischemia-reperfusion damage is thus of critical importance. With pluripotent bioactivities, the prot...

The protective effect of an L-type calcium channel blocker, nimodipine, on cell injury induced by oxygenglucose deprivation (OGD) in PC12 cells was investigated. PC12 cells were exposed to oxygen-glucose deprivation (30 minutes and 60 minutes respectively) in the presence or absence of nimodipine (10μM/L) in three different time schedules (pre-24h, pre-3h and concurrently). Cellular viability w...

Rabbit retinas were fixed for electron microscopy immediately after removing the eye and after incubations in a control medium and in three different deprivation media that were identical with the control except for the omission of glucose, oxygen, or both. A systematic comparison was made of the electron microscopic appearance of the different retinas with particular attention to four regions:...

Purpose. To examine the neuroprotective effects of neuronal nitric oxide synthetase (NOS) inhibitor in the development of ischemic retinal degeneration. Methods. In the rat ex vivo retinal preparations, simulated ischemia was induced by deprivation of oxygen or glucose from the incubation medium. GYKI (non-NMDA receptor antagonist) were applied to the retinal samples in combination with 7-NIA (...

The effects of raised brain lactate levels on neuronal survival following hypoxia or ischemia is still a source of controversy among basic and clinical scientists. We have sought to address this controversy by studying the effects of glucose and lactate on neuronal survival in acute and cultured hippocampal slices. Following a 1-h hypoxic episode, neuronal survival in cultured hippocampal slice...