Background: This study was carried out to assess the effects of MnTBAP, a cell permeable antioxidant, on motility, membrane integrity, capacitation status and in vitro fertilization ability of frozen-thawed ram semen. Materials and Methods: Fresh semen ejaculates were collected with artificial vagina from five rams, mixed and divided into five equal fractions, and diluted (1:20 v/v) with commer...

We report here a method for enhancing the non-oxygen functionalization of graphene nanosheets. This improvement is taken place by increasing oxygen functional groups as active reaction sites on oxide cold plasma treatment followed substitution octadecylamine (ODA). The FTIR, XPS, XRD, RAMAN, and TGA analyses verified that modification could increase amount 61%. plasma-treated functionalized nan...

In order to protect the spermatozoa against cold shock, hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders for domestic animals. The protective action of yolk is largely presumed to be due to low density lipoproteins (LDL). The effects of LDL on sperm quality of bull and northern pike (Esox lucius) after freezing-thawing have been reported, but no study has been m...

Background: Intracytoplasmic sperm injection (ICSI) can be considered the solstice in vitro insemination technique because it has powerfully allowed the treatment of male factor infertility, but in this procedure, except visual morphological selection, there is no standardization for sperm selection. Recently, hypo osmotic swelling test (HOST) has been proposed to have the potential to select i...

Introduction: Synaptosomes are sealed particles that contain mitochondria, cytoskeleton and vesicles which are necessary to synaptic events like neurotransmitter release and uptake in the nervous system. However, the effect of high and low temperatures on synaptosome membrane integrity and function during a time course after its extraction is less known. The purpose of this study was to assess ...

the aim of this study was to investigate the effect of adding different levels of zinc sulfate in semen extender on bovine sperm quality during cryopreservation. semen was collected from 4 holstein bull twice a week and the proper samples were pooled. semen were allocated to four extender treatments consisted of 0, 50, 100, 150 µm of zinc sulfate in 5 replicates and then, were frozen. post-thaw...

Background The aim of present study is to evaluate the effect of PolyVinyl Pyrrolidone (PVP) routinly used during ICSI procedure on sperm membrane integrity, and sperm chromatin status. MaterialsAndMethods This study was carried out on 21 semen samples from the infertile men referring to Isfahan Fertility and Infertility Center. The processed semen samples were divided into two portions. One po...

Plasma membrane H+-ATPase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. To study the effect of NaCl on the expression pattern of a gene encoding the plasma membrane H+-ATPase, an experiment was carried out in a completely random design with three replications. A pair of specific primers was designed based on the sequence o...