the effect of zinc nano-particles (zn- nano- complex) on bull sperm quality after freeze-thawing process studied. ejaculates collected from four holstein bulls twice a week. on the day of semen collection, four ejaculates were pooled and diluted with bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc nano-complex and frozen. after thawing, sperm motility and motion param...

The aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. In this experiment, different concentrations of glutathione [0 (LG-0), 0.5 (LG-0.5), 1 (LG-1), 1.5 (LG-1.5), and 2 (LG-2) mM] were added in a soybean lecithin-based extender. A total of 16 ejaculates from four bucks were collected and pooled. Each pooled sample was divided into five...

the aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. in this experiment, different concentrations of glutathione [0 (lg-0), 0.5 (lg-0.5), 1 (lg-1), 1.5 (lg-1.5), and 2 (lg-2) mm] were added in a soybean lecithin-based extender. a total of 16 ejaculates from four bucks were collected and pooled. each pooled sample was divided into five...

background: cryopreservation of semen requires optimized conditions to minimize the harmful effects of various stresses. the main approach for protection of sperm against stress is based on the use of antioxidants and cryoprotectants, which are described as defensive methods. recently, the application of controlled mild stressors has been described for activation of a temporary response in oocy...

the goal of this study was the evaluation of semen extender filtration on post-thawed buck sperm quality. a total 16 ejaculates were collected from four buck (4 ejaculates from each buck) twice a week. after semen collection and primary evaluation, collected semen samples were pooled and divided into two equal parts. each part was diluted with one of the following extenders: 1) non-filtered lec...

Adding antioxidants to semen extenders could reduce post-thaw sperm damages. Glutathione is an important intracellular antioxidant that has free radicals scavenging properties and multiple effects on DNA and protein synthesis. In the present study, effects different levels of reduced glutathione (GSH) containing 0 (control), 1.0, 2.5, or 5.0 mM in a nanomicelle-based extender on post-thaw quali...

background: exposure to arsenic is associated with impairment of male reproductive function by inducing oxidative stress. silymarin with an antioxidant property scavenges free radicals. objective: the aim of this study was to investigate if silymarin can prevent the adverse effects of sodium arsenite on ram sperm plasma membrane and acrosome integrity. materials and methods: ram epidydimal sper...

Background Cryopreservation of semen requires optimized conditions to minimize the harmful effects of various stresses. The main approach for protection of sperm against stress is based on the use of antioxidants and cryoprotectants, which are described as defensive methods. Recently, the application of controlled mild stressors has been described for activation of a temporary response in oocyt...

Chicken seminal plasma contains a high content of sex steroids including progesterone (P4). According to a published study, in vitro supplementation of P4 to the semen resulted in decreased fertility in the White Leghorn chicken; however, it is not known if increased P4 production in vivo is associated with decreased sperm fertility. Therefore, the aim of the present experiment was to study the...

the aim of this study was to evaluate the effect of bovine serum albomin (bsa) in stallion semen extender in cool condition. after collection, semen samples were pooled and divided into four equal parts. each part was diluted with one of the following extenders: 1) extender without bsa (bsa-0), 2) extender containing 5 mg/ml bsa (bsa-5), 3) extender containing 10 mg/ml bsa (bsa-10), and 4) exte...