Two kinds of prophage deletions of the Salm.onella phage P22 were isolated. One type of deletion was isolated by simultaneously selecting for loss of a host gene (pro) adjacent to the integrated prophage and for survival at the nonpermissive temperature of a temperature-inducible (tse2) lysogen. The second type of deletion was isolated by transduction to tetracycline resistance using high-frequ...

Fibroblasts from two affected members of a large pedigree in which osteogenesis imperfecta (01) type IV is genetically linked to the proa2(I) gene of type I collagen synthesize two populations of proa2(I) chains. One population is normal; the second population appears to have a deletion of about 10 amino acid residues from the middle of the triple helical domain. The mutation in proa2(I) causes...

A highly linear down conversion mixer with moderate gain used for 2.4GHz RF applications is presented in this paper using 0.18μm CMOS technology. The proposed mixer incorporates current bleeding technique & degenerative resistors to improve linearity and conversion gain. Also the negative feedback configuration of the circuit gives flat conversion gain increasing the overall bandwidth. The simu...

background: the ability of polyclonal antibodies to react with many epitopes of an antigen makes them valuable reagents in research and diagnosis. the aim of this study was purification of mouse igg2a and production of polyclonal antibody against purified mouse igg2a subclass. materials and methods: mouse igg2a was purified by proa affinity. verification method of the purified antibody was sds-...

Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 12, No. 10, October 2006 1607 biologic results. Since both strains were identified as L. pneumophila serogroup 1, we performed sequencebased typing (SBT) (3) of 6 genes (flaA, pilE, asd, mip, mompS, and proA) by using the protocol and database of EWGLINET. Both isolates showed identical SBT patterns (2,3,18,15,2,1). Isolates from 4 patients ...

The moderately halophilic bacterium Halobacillus halophilus copes with the salinity in its environment by the production of compatible solutes. At intermediate salinities of around 1 M NaCl, cells produce glutamate and glutamine in a chloride-dependent manner (S. H. Saum, J. F. Sydow, P. Palm, F. Pfeiffer, D. Oesterhelt, and V. Müller, J. Bacteriol. 188:6808-6815, 2006). Here, we report that H....

1. The growth of Escherichia coli proline auxotrophs on medium containing L-proline (50 microgram/ml) induces catabolic enzymes. A bioradiological assay system for proline, using proB cells of E. coli, might give erroneous results owing to proline catabolism by the proline auxotrophs on which the assay depends. 2. Differential utilization of proline and 1-pyrroline-5-carboxylate by the proB cel...

and N-body calculations (e.g., Ida 1990, Ida and Makino 1992b). It is shown that dynamical friction is effective in We investigate the growth and the orbital evolution of proa Keplerian potential as well as in a free space. Kokubo toplanets embedded in a swarm of planetesimals using threedimensional N-body simulations. We find that among protoplaand Ida (1996), hereafter referred to as Paper I,...

The data presented here reveal a new facet of the physiological adjustment processes through which Bacillus subtilis can derive osmostress protection. We found that the import of proteogenic (Glu, Gln, Asp, Asn, and Arg) and of nonproteogenic (Orn and Cit) amino acids and their metabolic conversion into proline enhances growth under otherwise osmotically unfavorable conditions. Osmoprotection b...