Auxotrophic proA mutants of Escherichia coli were complemented by two different classes of Corynebacterium glutamicum genes. One of these was the asd gene. The E. coli asd gene also complements the same proA alleles. Complementation of proA by the asd+ gene requires a high asd dosage and the proB and the proC gene products. The reciprocal complementation pattern (asd by the proA+ gene) was not ...

A 2.9 kb DNA fragment carrying the Escherichia coli proBA region, which encodes the first two enzymes of the proline biosynthetic pathway, was subcloned onto an expression plasmid carrying both the bacteriophage lambda PL promoter (lambda PL) and the lambda gene encoding a thermolabile cI repressor protein (cI857). Derepression of the lambda PL promoter by thermal inactivation of the cI857 repr...

A simple chromatographic procedure has been devised to separate gamma-glutamyl phosphate reductase and 1-pyrroline-5-carboxylate reductase, allowing the measurement of the former in crude Escherichia coli extracts. Analysis of a number of strains of E. coli has demonstrated that gene proA codes for gamma-glutamyl phosphate reductase and proB for gamma-glutamyl kinase. Introduction of a ColE1 hy...

The gamma-glutamyl-phosphate reductase (ProA) interlinks both the anabolic and osmostress adaptive proline biosynthetic routes of Bacillus subtilis. Because no paralogous protein to ProA exists in this microorganism, proA mutants should exhibit a tight proline auxotrophic growth phenotype. Contrary to expectations, proA mutants formed microcolonies on agar plates lacking proline and faster grow...

Introducción: No obstante la baja frecuencia de infecciones bacterianas asociadas al COVID-19, prevalencia del uso antibióticos empíricos es 70 a 90%. El objetivo primario estudio fue evaluar el impacto implementación un programa optimización antimicrobianos dirigido pacientes con COVID-19 (PROA-COVID).
 Material y métodos: Estudio antes después, retrospectivo, descriptivo analítico. Se ev...

Introducción: El uso de los antibióticos ha logrado curar infecciones anteriormente letales. Como profilaxis mejorado el pronóstico pacientes en múltiples escenarios; sin embargo, estos fármacos tienen efectos adversos cuales pueden ocurrir 1 cada 5 hospitalizados que reciben, algunos eventos son potencialmente mortales. Los programas Antimicrobial Stewardship (ASP), conocidos español como Prog...

A 3.6 kb DNA fragment carrying the Thermus thermophilus proBA region, which encodes the first two steps in the proline biosynthetic pathway, was cloned from the Thermus thermophilus gene library, and its complete nucleotide sequence was determined. The deduced amino acid sequence of gamma-glutamyl kinase (40,657 Da), the product of proB gene, and gamma-glutamyl phosphate reductase (48,747 Da), ...

Real-time and label-free antibody screening systems are becoming more popular because of the increasing output of purified antibodies and antibody supernatant from many antibody discovery platforms. However, the properties of the biosensor can greatly affect the kinetic and epitope binning results generated by these label-free screening systems. ForteBio human-specific ProA, anti-human IgG quan...

Introducción: Los programas de Optimización Antimicrobianos (PROA) en Instituciones Sanitarias son una estrategia implementada diversos países. El estudio: Nuestro objetivo fue realizar encuesta electrónica los establecimientos salud que cuentan con servicios hospitalización el Perú. Hallazgos: Ciento setenta (38.4%) respondieron la entre MINSA (90%), ESSALUD (4.2%), FFAA - PNP (2.9%) y Privado...