SH3 binding peptides contain polyproline helices and are classified according to their binding orientations as N-to-C-terminal or C-to-N-terminal. We have tested the hypothesis that such a peptide binds in both orientations but with different populations. A focal adhesion kinase (FAK)-derived peptide was tested for its binding orientation on the Src SH3 domain. Paramagnetic tags were introduced...

Lin et al. examined the effects of lipid chains attached to N-terminal antimicrobial peptides, a type membrane-active on their membrane activity and bioactivity. The impact lipidation varied depending original peptides' hydrophobicity. By altering peptide conformations affecting self-aggregation behaviours, changed peptide-biomembrane interaction, ultimately bioactivity (e329).

Objective: Hyponatremia is the most common electrolyte imbalance. The initial treatment decision is based on clinical evaluation of patient volume status but an accurate assessment is difficult, particularly differentiating mild hypovolemia from euvolemia. The aim of this study is to examine if biomarkers are valuable in the early determination of volume status and SIADH diagnosis. Methods: Blo...

AIMS Cardiac peptides have diagnostic and prognostic value in heart failure. Their plasma concentrations, however, are sensitive to rapid changes in haemodynamics. As blood sampling under standard conditions is not feasible in clinical practice, it is important to know which peptides are most resistant to change. Therefore, the present study investigated the differences in response to exercise ...

The present study was aimed to construct a fusion plasmid harboring the extracellular domain of the influenza A M2-protein (M2e), which was fused to the N-terminus of the truncated HSP70 (HSP70359–610) molecule as a new approach for future vaccine research against influenza A. The amplified fragments, M2e and HSP70359-610 genes, were gel-purified. The products were then single digested with Bam...

Background and Purpose: Several clinical scoring systems as well biomarkers have been proposed to predict stroke-associated pneumonia (SAP). We aimed externally competitively validate SAP scores hypothesized that 5 selected would improve performance of these scores. Methods: pooled the data 2 acute stroke studies with identical assessment: STRAWINSKI PREDICT. Biomarkers (ultrasensitive procalci...